大鼠纹状体边缘区内烟碱型乙酰胆碱受体mRNA的表达

摘要

目的研究纹状体边缘区内有无乙酰胆碱受体(nAChR)的分布。方法用地高辛标记的nAChRcDNA作为探针，与大鼠脑切片进行原位杂交。结果大鼠脑纹状体内可见较多的nAChR阳性神经元，其中尾壳核中可见较多中等大小的多极阳性神经元，在尾壳核和苍白球之间的边缘区部位，可见较多阳性梭形细胞，呈密集的带状分布。结论纹状体边缘区内存在nAChRmRNA的表达，推测边缘区内的nAChR阳性神经元对调节边缘区的学习记忆功能有重要作用。%Objective To study the distribution of nicotinic acetylcholinereceptor（nAChR） mRNA in the marginal region of rat striatum. Methods In situ hybridization of digoxin-labeled probes on sections of the rat brain was performed. Results Numerous nAChR-positive neurons were observed in the rat striatum. In the caudoputamen aggregated many multipolar positive neurons of moderate size and a number of fusiform positive neurons were present in the caudoputamen.There were some fusiform positive neurons in the marginal division,which were located in the marginal region between the caudate putamen and globus pallidus. Conclusion The neurons in the marginal region are capable of nAChR mRNA expression, suggesting that nAChR-positive neurons in the marginal region may exercise important functions in the process of learning and memory.