Objective To investigate apoptosis of tumor infiltrating dendritic cells (TIDC) and their expression of Fas/ FasL (CD95/CD95L) in human endometrioid adenocarcinoma. Methods The apoptotic rate of TIDC was measured in 45 cases of endometrioid adenocarcinoma and 20 cases of normal endometrium tissues (control) by double-label immunohistochemistry using the monoclonal antibody S-100 protein and TUNEL technique. The expressions of Fas and FasL in TIDCs were detected using double-label immunohistochemistry and imaging analysis. Results The apoptotic rate of TIDCs in endometrioid adenocarcinoma were significantly higher than that in normal endormetrium [(13.02±0.64)% vs (6.82±0.53)%, P＜0.05]. The expression levels of Fas in the TIDCs were significantly lower, whereas FasL expression significantly higher in endometrioid adenocarcinoma than in normal endormetrium (7.88 ±1.05 vs 19.25 ±3.03, P＜0.05; 12.95 ±2.25 vs 7.51 ±1.14, P＜0.05). Conclusion Increased apoptosis of the TIDCs and abnormal expression of Fas/FasL in TIDCs in endometrioid adenocarcinoma may lead to tumor immune escape.%目的 探讨子宫内膜癌浸润性树突状细胞(TIDC)凋亡及其凋亡相关蛋白Fas/FasL的表达意义.方法 利用S-100单克隆抗体和DNA缺口末端标记(TUNEL法)相结合,分析45例子宫内膜癌组织中TIDC凋亡状况；通过免疫组织化学双重染色方法检测TIDC表面Fas/FasL分子表达.结果 子宫内膜癌组织中TIDC凋亡发生率显著高于子宫内膜组织中树突状细胞凋亡发生率[(13.02+0.64)%vs(6.82+0.53)%,P＜0.05]；子宫内膜癌组织TIDC膜表面Fas表达显著低于正常子宫内膜组织(7.88±1.05vs19.25+3.03,P＜0.05),子宫内膜癌组织TIDC膜表面FasL表达显著高于正常子宫内膜组织(12.95±-2.25vs7.51±-1.14,P＜0.05).结论 子宫内膜癌组织中TIDC凋亡增加及Fas、FasL在TIDC上表达失衡,可能导致子宫内膜癌免疫逃逸发生.
展开▼
