首页> 中文期刊>南方医科大学学报 >信号传导和转录激活因子3及其蛋白抑制剂在狼疮小鼠肾组织中的表达

信号传导和转录激活因子3及其蛋白抑制剂在狼疮小鼠肾组织中的表达

     

摘要

Objective To investigate the expression of STAT3 and PIAS3 in the renal tissues of rats with lupus nephritis.Methods The kidneys were harvested from 18-week-old female MRL/lpr mice (lupus nephritis model group) and age-matched female BALB/C mice (normal control group).The expressions of STAT3 and PIAS3 mRNA in the renal tissues were measured by real-time quantitative RT-PCR,and the protein levels of STAT3 and p-STAT3 were examined using Western blotting and immunohistochemistry.The laboratory indices and renal histopathology of the mice were also investigated.Results The urinary protein,blood urea nitrogen (BUN) and creatinine levels were significantly higher in MRL/lpr mice than in the control mice (P<0.05).The renal histopathology of MRL/lpr mice was characterized by increased mesangiocapillary proliferation and prominent inflammatory infiltration in the tubulo-interstitium,which were absent in the kidneys of normal control mice.Compared with the control mice,MRL/lpr mice showed significantly increased STAT3 mRNA and p-STAT3 protein levels in the renal tissues (P<0.05) with significantly lowered levels of PIAS3 mRNA (P<0.01).No significant difference was noted in the total STAT3 protein levels between MRL/lpr and control mice.Conclusion MRL/lpr mice have significantly increased expressions of STAT3 mRNA and p-STAT3 protein and decreased expression of mRNA PIAS3 in the kidneys compared with BALB/C mice.%目的 探讨信号传导和转录激活因子3(STAT3)及其蛋白抑制剂(PIAS3)在MRL/lpr狼疮小鼠肾组织中的表达.方法 以18周龄雌性MRL/Ipr小鼠作为狼疮肾炎组,相同周龄的BALB/C小鼠作为正常对照组.分别取两组小鼠肾组织,应用荧光定量RT-PCR法检测STAT3和PIAS3 mRNA的表达,采用Western blot和免疫组织化学方法检测磷酸化STAT3(p-STAT3)和STAT3的蛋白表达,司时检测小鼠实验室指标以及肾脏组织病理的表现.结果 MRL/Ipr小鼠尿蛋白、血尿素氮、血肌酐均高于BALB/C小鼠(P<0.05).MRL/Ipr小鼠肾脏组织病理主要表现为肾小球系膜细胞增生,肾小管间质炎症细胞浸润,而BALB/C小鼠肾小球、肾小管无异常.MRL/lpr小鼠肾组织STAT3 mRNA和p-STAT3蛋白的表达均较BALB/C小鼠升高(P<0.05),其PIAS3mRNA表达则较BALB/C小鼠明显降低(P<0.01),而两组小鼠STAT3总蛋白的表达无明显差别.结论 MRL/lpr小鼠肾组织STAT3 mRNA和p-STAT3蛋白的表达均高于对照组,而其PIAS3 mRNA表达低于对照组.

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