Objective To characterize the effect of omeprazole on the spectrum of gene expression in the cultured human umbilical vein endothelial cell (HUVEC) line (EA.hy926),and explore the underlying molecular mechanism.Methods Affymetrix U133 plus2.0 oligonucleotide microarray was used to detect the alteration in the gene expression profiles induced by l×10-5 mol/L omeprazole in HUVECs.Real-time PCR was employed to verify the results of selected differentially expressed genes,and Western blotting was performed to test the expression levels of the related proteins.Results A total of 282 genes were found to show at least 1.5-fold changes in EA.hy926 cells after treatment with omeprazole for 48 h,including 236 up-regulated and 46 down-regulated ones.These genes were involved in the regulation of transcription,inflammatory response,immune response,cell adherence,anti-apoptosis,and signal transduction.Conclusion Omeprazole modulates the function of endothelial cells by regulating the gene expression profiles of multiple pathways.%目的 研究奥美拉唑对体外培养人脐静脉内皮细胞(EA.hy926)基因表达谱的影响,探讨奥美拉唑对内皮细胞作用的分子机制.方法 10-5mol/L奥美拉唑与人脐静脉内皮细胞株共培养48h,运用Affymetrix U133 plus 2.0全基因组表达芯片,检测奥美拉唑对人脐静脉内皮细胞基因表达谱的影响,分子注释系统MAS3.0软件进行聚类分析.RT-PCR验证基因表达谱结果,Western blotting验证相关蛋白水平的变化.结果 奥美拉唑作用内皮细胞48h后,基因芯片分析筛选出差异表达大于1.5倍的基因282个,其中上调基因236个,下调基因46个,包括转录调节、炎症反应、免疫反应、细胞粘附、抗凋亡、信号转导等相关基因,RT-PCR和Western blotting结果与基因芯片结果一致.结论 奥美拉唑在基因水平通过多条通路调节内皮功能.
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