上皮性卵巢癌中CD133与EMT相关因子表达的关系

摘要

Objective To explore expressions of CD133, E-cadherin and Snail in hu¬man epithelial ovarian cancer (EOC) and elucidate their relationship with the clinicopathologic features and prognosis of the patients. Methods The expression of CD133, E-cadherin and Snail were detected by immunohistochemical staining in 150 specimens of EOC and 50 specimens of benign ovarian epithelial tumor tissues. Results The positivity rates of CD133, E-cadherin and Snail protein in EOC were 58.7%, 60.7%and 32.7%, respectively, significantly different from the rates in benign epithelial tumor tissues (10%, 8.0%, and 70%, respectively; P<0.05). The expressions of CD133, E-cadherin and Snail in EOC were significantly correlated with abdominal organ and lymphnode metastases and FIGO stage (P<0.01). E-cadherin expression was inversely correlated with Snail and CD133 expression (r=-0.545 and-0.570, P<0.01), and the latter two were positively correlated (r=0.599, P<0.01). Overexpressions of CD133 and Snail and a decreased expression of E-cadherin were all related to a poor prognosis of the patients (P<0.05). FIGO stage and expressions of CD133, E-cadherin and Snail were all independent prognostic factors of EOC (P<0.05). Conclusion The expressions of CD133, E-cadherin and Snail are related to lymph node metastasis, clinical stage, and prognosis of EOC. Combined detection of these indexes provides important evidence for predicting the progression and prognosis of EOC.%目的：探讨上皮性卵巢癌（Epithelial ovarian cancer, EOC）中CD133蛋白及上皮-间质转化（Epithelial mesenchymal transition, EMT）相关因子Snail、E-cadherin蛋白表达的关系及其功能意义。方法采用免疫组织化学ElivisionTM plus法检测150例EOC和50例良性上皮性卵巢肿瘤中CD133、Snail和E-cadherin蛋白的表达情况。结果良性上皮性卵巢肿瘤组织中CD133、Snail和E-cadherin蛋白的表达阳性率分别为10%、8.0%和70%，在EOC组织中3者表达分别为58.7%、60.7%和32.7%，差异有统计学意义（P<0.05）。CD133、Snail和E-cadherin的表达与EOC的腹腔脏器和淋巴结转移以及FIGO分期有关（P<0.01）；E-cadherin蛋白的表达与Snail及CD133蛋白的表达均呈负相关关系（r分别为-0.545和-0.570，P均<0.01），CD133蛋白的表达与Snail蛋白的表达呈正相关关系（r=0.599，P<0.01）。Kaplan-Meier生存分析表明，CD133和Snail的过表达均与患者的生存率有关，阳性表达的患者生存率明显低于阴性者（P<0.05）；而随着E-cadherin蛋白表达水平的降低EOC患者生存率明显下降（P<0.05）。多因素分析表明FIGO分期、CD133、Snail和E-cadherin的表达是影响EOC根治术后患者预后的独立因素（P<0.05）。结论 CD133和Snail表达的升高以及E-cadherin表达的降低与EOC的侵袭、转移和预后等因素相关，这些指标的联合检测有可能作为评估EOC患者临床预后的重要指标。