波兰、英国油菜种子样本的健康测试及黑胫病病原菌感染分析

摘要

对来自波兰及英国的2个相同品种的油菜种子样品进行了健康测试,并重点观察了油菜黑胫病病原菌(Letosphaeria maculans/ Phoma lingam)感染的程度及菌系类型.结果表明来自2个国家的种子都一定程度受微生物感染,品种间差异不大,所检测到的较多的菌属为Alternaria,Fusarium及Penicillium.黑胫病病原菌感染的百分率波兰种子样品平均为0.5%,英国种子样品平均为0.6%,菌系鉴定表明,7.1%的波兰株系及12.3%的英国株系为强致病的Tox+类型.此外,还发现健康测试前种子表面消毒可提高黑胫病病原菌的检出率.%Mycological analysis and evaluation of Leptosphaeria maculans(Phoma lingam) contamination in Brassica napus seed samples from England and Poland were conducted. The results showed that 26%～40% of the seeds were contaminated by fungi and bacteria with Alternaria,Fusarium and Penicillium as the most commonly recovered genera. P. lingam contamination in Polish and English seed samples were 0.5% and 0.6% respectively, of which 7.1% of Polish isolates and 12.3% of English ones were classified as Tox+ pathotype. Seed surface sterilization prior to seed health testing resulted in an increase of the detection of P.lingam.