土壤改良对越橘根域细胞分裂素含量及年变化规律的影响

摘要

【目的】研究不同土壤改良物质对越橘Vaccinium vitis-idaea 根际土和非根际土中细胞分裂素[玉米素（ Z）、玉米素核苷（ ZR）和异戊烯基腺苷（ iPA）]含量及年变化规律的影响．【方法】以2年生盆栽越橘为试材，应用“根际微区土壤剥落分离法”收集与根系黏着程度不同的非根际土和根际土样品，用高效液相色谱法测定土壤样品细胞分裂素含量．【结果和结论】不同根域处理的根系分泌物中，玉米秸秆配施氮肥处理（ k4）的细胞分裂素含量最高，其他依次为添加玉米秸秆处理（k3）、添加草炭处理（k2）、无添加（k1，对照）；不同时期越橘根际土和非根际土中细胞分裂素含量总趋势为Z＞ZR＞iPA，如k4处理的Z、ZR和iPA平均质量比分别为9.89、9.76和3.57 ng／g，分别比k1（对照）高出67.9％、84.2％和105.6％．越橘根系细胞分裂素含量年变化规律表现为早春时期呈上升趋势，秋末季节含量呈下降趋势，而在早春至秋末之间，根系分泌物含量较高．%Objective] The contents and annual variations of cytokinins including zeatin (Z), zeatin ri-boside(ZR) and isopentenyl adenodine(iPA) were studied in the rhizosphere and non-rhizosphere of blueberry, Vaccinium vitis-idaea‘Northland’ after the application of different soil amelioration materials in pots.[Method] Rhizosphere and non-rhizosphere soil samples with different root adhesion degrees were collected by rhizosphere soil stripping separation method ,high performance liquid chromatography meth-ods were used to investigate cytokinin contents in soil samples. [Result and conclusion] Cytokinin con-tents of corn stalks mixed with nitrogen fertilizer(k4)were the highest, followed by the application treat-ments of corn stalks(k3), peat(k2), and no added (k1,control).The general trend of cytokinin contents in blueberry rhizosphere and non rhizosphere soil were Z >ZR>iPA in different periods, such as k4 treat-ment the Z, ZR and iPA average content was 9.89, 9.76 and 3.57 ng/g respectively, which were higher than 67.9%,84.2%and 105.6%compared with the control, respectively. The annual variations of cyto-kinins in the root zone of blueberry were traced. The contents of cytokinins increased in early spring and declined in autumn, while between in early spring and late autumn they maintained high levels.