拟南芥细胞分裂素受体AHK3亚细胞r定位信号的研究

摘要

[Objective]To study the subcellular localization signals of cytokinin receptors and related mechanisms.[Method]A series of expression vectors for subcellular localization of Arabidopsis histidine protein kinase 3 (AHK3), one of cytokinin receptor proteins in Arabidopsis, were constructed and transformed into Arabidopsis protoplast cells for transient expression. Laser confocal microscope was used to observe the subcellular localization of AHK3 protein.[Result]AHK3 is localized in endoplasmic reticulum(ER). Both N and C terminals contain ER localization sequences.[Conclusion]AHK3 carry out its functions in ER, such as perception of cytokinin, downstream signal transduction and interactions with other receptor proteins, and AHK3 contains multiple ER localization signals.%[目的]研究细胞分裂素受体在细胞内定位分布的具体信号及其机制.[方法]构建拟南芥细胞分裂素受体蛋白——组氨酸蛋白激酶3(Arabidopsis histidine kinase 3,AHK3)的一系列亚细胞定位相关表达载体,转化到拟南芥原生质体细胞中进行瞬时表达后,采用激光共聚焦显微镜观察研究AHK3的亚细胞定位信号.[结果]AHK3定位于内质网(Endoplasmic reticulum,ER);AHK3的N端和C端均含有ER定位序列.[结论]AHK3在ER中实现对细胞分裂素的感知及受体蛋白之间的相互应答,并进行下游的信号转导;AHK3含有多段ER驻留信号.