SCH9基因对酿酒酵母中H2S代谢的影响

摘要

Hydrogen sulfide(H2S)is the endogenous signaling molecule and has attracted great attention for its significant role in curing cardiovascular nervous system diseases.Firstly,we surveyed the effect of SCH9 gene deletion on H2S production in SCH9 deletion yeast strains RCD398,RCD399 and TS120-2d by quantifying H2S levels using lead acetate paper.WT(BY4741)yeasts produced H2S of 4(mm)/A600nmafter cultured for 48 h.While Sch9Δ(RCD398)only produced H2S of 0.4 mm /A600nmwithout caloric restriction.Under caloric restriction,WT(BY4742)yeasts produced H2S of 10(mm)/A600nm after cultured for 48 h,whereas H2S was not detected in Sch9Δ(RCD399)yeasts.Secondly,we studied the effect of SC H9 gene deletion on exogenous H2S degradation by substitute GYY4137 and NaHS as external H2S donors.H2S wasn't detected in WT(BY4742)strain after cultured for 72 h,while re-mained to be detected in Sch9Δ(RCD399)strain.These results suggest that SCH9 deletion decreases synthesis and catabolism of H2S in yeast.%H2S作为胞内气体信号分子,在心血管和神经系统方面的治疗显著效果引起了人们的关注.首先,为了探究酿酒酵母中 SC H9基因缺失对其 H2S生成的影响,我们以酿酒酵母 SCH9基因缺失型菌株RCD398、RCD399和TS120-2d为研究对象,用醋酸铅试纸检测反应体系中H2S含量.非热量限制条件,WT(BY4741)培养48 h 产生了4(mm)/A600nm H2S,而 Sch9Δ (RCD398)只产生0.4(mm)/A600nm.热量限制条件下,WT(BY4742)培养48 h产生了10 (mm)/A600nm的H2S,而 Sch9Δ(RCD399)未检测到H2S.其次,为了探究SCH9基因缺失菌株对外源H2S分解的影响.用GYY4137和NaHS作为H2S的供体.WT(BY4742)培养72 h之后未检测到H2S,而Sch9Δ(RCD399)仍可持续检测到大量H2S.结果表明 SCH9缺失既可以下调酿酒酵母中H2S的生成,也可以下调酿酒酵母对外源H2S的分解.