为进一步探讨金边百里香植株内挥发性物质生物合成的组织特异性及其机理，以1年生金边百里香（Thy⁃mus mongolicus Ronn）幼苗为试验材料，采用顶空固相微萃取（SPME）结合气相色谱-质谱联用（GC-MC）技术对金边百里香叶、侧枝、主枝和根的挥发性成分进行了检测，并用峰面积进行归一化定量。结果表明，金边百里香不同部位的芳香成分及其含量差异显著，叶、侧枝、主枝、根中分别检测出32，35，31，28种挥发性物质，其含量分别为各部位总挥发性物质的99.03%，99.33%，79.98%，91.30%。金边百里香叶、侧枝、主枝、根中有6种共有成分，分别为邻异丙基甲苯、丙烯酸异冰片酯、莰烯、4-萜烯醇、NA、十一烷，其中，邻异丙基甲苯为最主要的挥发性成分。此外，有差异组分的化合物种类少且相对含量也比较低。%To lay the foundation of further explore the plant volatile compounds in biosynthesis of tissue specificity and mechanism of Thymus mongolicus Ronn, this paper took one-year-old Thymus mongolicus Ronn seedings as materials, adopted the solid-phase micro-extraction(SPME)and gas chromatography mass spectrometry(GC-MS)to determination the volatile compounds of the leaf, lateral branch, main branch, root of Thymus mongolicus Ronn, and analyzed quantitatively by area normalization. The results showed that the aromatic components and the content of different parte of Thymus mongolicus Ronn were significant difference, 32, 35, 31 and 28 chemicals were identified by GC/MS correspondingly from the leaf, lateral branch,main branch,root of Thymus mongolicus Ronn, the identified components accounted for 99.03%, 99.33%, 79.98% and 91.30% of the total volatile compounds. The common constituents of the volatile compounds of the leaf, lateral branch,main branch,root of Thymus mongolicus Ronn were p-Cymene, Isobornyl acrylate, Camphene, Terpinen-4-ol, NA, Undecane. Among, the p-Cymene was the main volatile compounds of Thymus mongolicus Ronn. In addition, less species were different components of compound and the relative content was lower.
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