EFFECTS OF ANTI- PML/RARα OR ANTI- PML ANTISENCE ON THE GROWTH, DIFFERENTIATION AND APOPTOSIS OF NB4 CELL LINES

摘要

Objective To investigate the effects of anti- PML/RARα or anti- PML antisence on the growth,differentiation and apoptosis of NB4 cell lines. Methods Wright's stain for cell morphology, flow cytometry and DNA gel electronphoresis for cell apoptosis, methylcellulose assays for leukemic colony forming unit and trypan - blue exclusion for cell counts. Results Both the start cordon region of the PML or PML- RARα mRNA(STAS) and the fusion point region of the long type PML - RARα mRNA (FUAS) could inhibit cell growth. Cells became partially differentiated at 5d of treatment, and FUAS- treated cells showed more significant differentiation than STAS-treated cells. Morphology of typical apoptosis could be seen at 7, 9d incubation with antisence oligodeoxynucleotides (AS). In contrast, no cell growth inhibition, no morphology changes were seen in Sen or Ran treated cells compared with untreated cells. The number of acute myelocytic leukemia colony forming unit(AML - CFU) markedly decreased in STAS and FUAS treated cells. Cell DNA content analyzed by flow cytometry showed the typical profile of apoptotic cells, in which pre- G1 peak appear before G1 peak at 7,9d of treatment with STAS or FUAS. Conclusion Anti-PML/RARα or anti-PML antisence inhibit the cell growth, induce differentiation and differentiated cell apoptosis of NB4 cells.