去唾液酸糖蛋白受体在乙型肝炎病毒感染者精子中表达的初步研究

摘要

目的 检测乙型肝炎病毒(HBV)感染者精子中去唾液酸糖蛋白受体(ASGP-R)的表达,并与HBV阴性男性精子比较,探讨ASGP-R是否参与介导HBV侵入人精子.方法 用ASGP-R单抗,通过免疫印迹(Western blot)和流式细胞术检测ASGP-R在乙肝表面抗原(+)/乙肝e抗原(+)[HBsAg(+)/HBeAg(+)]者(双阳性组,n=16)、HBsAg(+)/HBeAg(一)者(单阳性组,n=28)和HBsAg(-)者(阴性组,n=35)精子中表达差异.结果 ASGP-R于人精子的胞质中高表达,于胞膜低表达；胞质中ASGP-R水平在阴性组、单阳性组及双阳性组中逐渐升高,双阳性组和单阳性组的胞膜上ASGP-R的阳性率均高于阴性组(P均＜0.05).结论 人精子胞膜和胞质中均存在ASGP-R; HBV可能通过人精子表面的ASGP-R进入精子,且HBV可能通过某种机制使ASGP-R的表达量增加.%Objective: To explore the mechanism of hepatitis B virus (HBV) invading into human sperm through detecting and comparing the expression of the asialoglycoprotein receptor( ASGP-R) in spermatozoa of HBV Infected male patients and the HBV-negative men.Methods: The expression levels of ASGP-R in HBV negative group(? = 35),single-positive (HBsAg + /HBeAg-) group (w = 28)and double-positive (HBsAg+/HBeAg+) group (m= 16)were detected with ASGP-R monoclonal antibodies by Western blot and flow cytometry.Results: ASGP-R existed in the plasma membrane and the cytoplasm of human sperm. ASGP-R was highly expressed in human sperm membrane and lowly expressed in human sperm cytoplasm. The expression levels of ASGP-R in negative group, single-positive group and double-positive group were increased gradually. There were significantly higher ASGP-R positive rates in single-positive group and double-positive group than that in negative group (P<0. 05).Conclusions: There was ASGP-R expression in the plasma membrane and the cytoplasm of human sperm. HBV might invade into the human sperm by ASGP-R which exist in the plasma membrane. HBV might increase the expression levels of ASGP-R by means of some mechanism.