子痫前期患者血清中抗血管紧张素Ⅱ1型受体抗体对大鼠小动脉功能的影响

摘要

Objective: To study the effect of autoantibody against angiotensin Ⅱ type 1 receptor (AT1-AA) in serum of preeclampsia patients on rat small vascular function (middle cerebral artery, coronary artery, mesenteric, renal and uterine arteries) , and investigate the role of AT1-AA in pathogenesis of preeclampsia.Methods: Vasoconstrictive effect of purified IgG from the sera of either preeclampsia patients or normal pregnant women on isolated small arteries of rat was determined.Results: The results showed that AT1-AA showed variant effects on small vascular rings isolated from different organs. Of these vessels, middle cerebral artery and coronary artery were remarkably contracted by AT1-AA. A dose of 1. 0 μmol/L AT1-AA increased vasoconstrictive tensile force of aortic middle cerebral artery and coronary artery vascular rings from(0. 03 ± 0. 02)mN to(0. 82 ± 0. 09)mN and(2. 51 ± 0. 19) mN respectively. The changes of tensile force of mesenteric, renal and uterine arteries were not significant.Conclusions; These findings suggest that ATI-AA is a novel risk factor in pregnant women and mightplay a causative role in the development of preeclampsia.%目的 采用微血管环技术,观察抗血管紧张素II 1型受体的自身抗体(AT1-AA)对主要脏器的小血管(包括冠状动脉、大脑中动脉、肾动脉、子宫动脉和肠系膜动脉)的直接作用,明确该自身抗体对子痫前期患者主要脏器的影响.方法 利用MAb Trap Kit试剂盒对子痫前期患者抗体阳性者血清中的抗体IgG进行提取和纯化,用血管环技术检测纯化后的抗体对大鼠小动脉收缩功能的影响.结果 AT1-AA浓度依赖性收缩大脑中动脉和冠状动脉,1.0μmol/L的AT1-AA可使大鼠大脑中动脉和冠状动脉的最大收缩张力从对照组的(0.03±0.02)mN分别增加到(0.82±0.09)mN和(2.51±0.19)mN,而对肾动脉、肠系膜动脉和子宫动脉的收缩作用不明显.结论 AT1-AA浓度依赖性地增强大鼠大脑中动脉和冠状动脉的收缩,可能是引起妊娠高血压综合征的原因之一.