头颈部鳞癌中TP53和NOTCH1基因的共表达网络及功能分析

摘要

Objective To find out the expression relation between TP53 and NOTCH1,and to explore their effects in head and neck squamous cell carcinoma.Methods Obtained the differentially expressed genes data of head and neck squamous cell carcinoma from 279 samples in TCGA database.Analyzed the co-expression relation between TP53 and NOTCH1 through Pearson and Spearman method.Cbioportal was used to analyze their co-expressed genes.Establish the co-expression network of TP53 and NOTCH1 with String database.The pathway and function of co-expression network was identified through KEGG and DAVID database respectively.Results Among the 279 samples,TP53 and NOTCH1 was co-expressed in head and neck squamous cell carcinoma.(Pearson score =0.45;Spearman score =0.41) There were 182 interaction pairs of TP53 and NOTCH1 related co-expressed gene according to the String database.(Pearson and Spearman score ＞ 0.3)These genes were enriched in some pathways such as T cell receptor signaling pathway,cell cycle,cell adhesion molecules and so on.These genes were enriched in some tumor related function including immune response,regulation of transposition,regulation of apoptotic process,cell cycle,regulation of GTPase activity and so on.Conclusion TP53 and NOTCH1 was co-expressed.Through establishing co-expressed network of TP53 and NOTCH1 and bioinformatics analysis,their function and signaling pathway were explored.The data generated from this study could provide a new reference in mechanism research of head and neck squamous cell carcinoma.%目的 探索TP53和NOTCH1基因在头颈部鳞癌中的表达关系及其作用.方法 选取TCGA数据库中的279例头颈部鳞癌二代测序样本,获得头颈部鳞癌中差异表达基因数据.利用Pearson和Spearman方法检测TP53基因和NOTCH1基因的共表达关系.刚用cbioportal筛选样本中与TP53和NOTCH1发生共表达的基因.利用String数据库,建立TP53-NOTCH1共表达网络.利用KEGG数据库分析共表达网络基因的富集通路.利用DAVID数据库分析共表达网络功能.结果 在TCGA数据库的头颈部鳞癌样本中,TP53和NOTCH1发生共表达.String数据库显示TP53和NOTCH1共有182对共表达基因的蛋白质互相作用对(Pearson分数=0.45;Spearman分数=0.41).KEGG分析显示这些共表达基因被富集在T cell receptor signaling pathway,cell cycle 和cell adhesion molecules等通路中(Pearson分数和Spearman分数均＞0.3).功能分析结果显示这些共表达基因的功能主要为免疫应答、转录调控、能量代谢、细胞周期调控和细胞凋亡调控等相关功能(P＜0.05).结论 TP53和NOTCH1在头颈部鳞癌中发生共表达;通过建立TP53-NOTCH1共表达网络和生物信息学分析,挖掘出二者在头颈部鳞癌中发挥的协同作用及其通路;上述结果能够为头颈部鳞状细胞癌的分子机制研究提供新的参考.