The aim is to study the serum iron changesin vitro serum of human peripheral blood irradiated by Co-60γ-rays and explore its feasibility as a biodosimetry. To establish the dose-response relationship, the concentration of serum iron inγ-rays irradiated serum was detected using ELISA (Enzymes linked immunosorbent assay). The results demonstrated that serum iron level increased with absorbed dose, and the dose-response curve of serum iron concentration followed the linear-quadratic model: male [y=30 676.5+2 383.5x–202.7x2 (r=0.99)], female [y=26 974.4+ 1 611.8x–141.3x2 (r=0.98)]. Time-response relationship of 4 Gy demonstrated that the serum iron was stable within 2 d. With the dose-response linear-quadratic curve, the absorbed dose was close to the double-blind dose (1, 2 and 4 Gy), and the 95% confidence interval of the estimated value was narrow. Therefore, the serum iron has the potential to be a new biodosimetry to reconstruct human exposure dose.%研究人体外周血血清经急性 γ 射线照射后血清铁的浓度变化,探讨其作为生物剂量计的可行性.人体血清经不同剂量γ射线辐照,采用酶联免疫吸附试验 (Enzymes linked immunosorbent assay, ELISA)法定量检测血清铁的浓度,建立血清铁浓度与吸收剂量之间的剂量?效应关系.结果显示,血清铁浓度随吸收剂量升高,血清铁浓度与吸收剂量成线性平方关系:男性[y=30 676.5+2 383.5x–202.7x2(r=0.99)];女性 [y=26 974.4+ 1 611.8x–141.3x2 (r=0.98)].4 Gy剂量时间?效应关系研究发现离体血清铁在2 d内维持稳定.利用建立的线性平方剂量?效应曲线估算的吸收剂量与双盲剂量(1、2、4 Gy)接近,估算值的95%置信区间较窄.研究结果提示血清铁具有重建人体吸收剂量的潜能.
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