不同浓度氧气对人未成熟卵子体外成熟和早期胚胎发育的影响

摘要

目的 收集辅助生殖治疗中废弃的人类未成熟卵子,研究其在不同浓度氧气培养条件下的卵子成熟率和受精及其后续早期胚胎发育的影响.方法 将收集的未成熟卵子随机分组为低浓度和高浓度氧气条件进行体外成熟培养,将成熟卵子进行卵胞质内单精子显微注射并行体外培养,研究卵子的体外成熟率、受精率以及胚胎卵裂率和优质胚胎率.结果 对于GV期来源卵子,低氧培养条件下的卵子体外成熟率和受精率要显著高于高氧组(P<0.05),两组间的胚胎卵裂率和优质胚胎率比较差异无统计学意义(P>0.05);MI期来源卵子在低氧培养条件下的卵子体外成熟率要显著高于高氧组(P<0.05),两组间的受精率、胚胎卵裂率和优质胚胎率差异无统计学意义(P>0.05).结论 低浓度氧气对于人类未成熟卵子的体外成熟有促进作用,能提高GV期卵子的体外成熟后的受精率;对于体外成熟培养卵子来源的胚胎卵裂和优质胚胎率没有影响.%Objective To determine the effects of oxygen at varied concentrations on in vitro maturation (IVM)of oocytes,and subsequent fertilization,early-stage development of embryos by collecting the human imma-ture oocytes from assisted reproduction treatment. Methods Immature oocytes were randomly allocated to be cul-tured with oxygen at a lower or higher concentration. Intracytoplasmic sperm injection of mature oocytes after IVM , the rates of maturation,fertilization,embryo cleavage and high quality embryo were investigated. Results The GV oocytes cultured with oxygen at the lower concentration yielded higher maturation and fertilization rates than those at the higher concentration(P<0.05). There were no significant differences in the rates of embryo cleavage and high quality embryo between two groups(P > 0.05). For MI oocytes,the maturation rate of oocytes cultured with oxygen at the lower concentration was higher as compared to that at the higher concertration (P < 0.05). There were no significant differences in the rates of fertilization ,embryo cleavage and high quality embryo between the two groups(P > 0.05). Conclusions Oxygen at a lower concentration is beneficial to IVM of human imma-ture oocytes and it also improves the fertilization of GV oocytes after IVM. Oxygen at a lower concentration has no beneficial effect on the embryo cleavage rate and high quality embryo rate.