补肾壮骨颗粒通过GH/IGF-1轴影响快速老化小鼠骨密度

摘要

目的 研究补肾壮骨颗粒是否通过提高快速老化小鼠(P6)局部骨组织GH/IGF-1轴的基因表达水平从而提高骨密度.方法 实验分4组:R1小鼠生理盐水灌胃组(R1组),P6小鼠分为生理盐水灌胃组(P6空组)、皮下rhGH组(rhGH组)和补肾壮骨颗粒灌胃组(补肾组),每组各10只,每日干预1次.分别干预3、6个月后进行骨密度测量和胫骨GH mRNA及IGF-1 mRNA表达水平检测.结果 干预3个月后,骨密度比较:R1组及补肾组高于P6空组;rhGH组与P6空组比较差异无统计学意义.GH mRNA和IGF-1 mRNA表达水平比较:R1组、rhGH组及补肾组均高于P6空组.干预6个月后,骨密度比较:rhGH组及补肾组较P6空组提高.GH mRNA和IGF-1 mRNA表达水平比较:GH组及补肾组较P6空组均有所上升.4组GH mRNA表达水平与IGF-1 mRNA表达水平呈正相关.P6各组GH mRNA、IGF-1 mRNA表达水平与全身各部位骨密度呈正相关.结论 补肾壮骨颗粒可以提高P6小鼠全身各部位的骨密度,其作用机制可能与提高局部骨组织GH mRNA与IGF-1 mRNA表达水平有关.%Objective To study whether the effects of bone mineral density by a kidney-tonifying herbal fufang treatment in senile osteoporosis mice (P6) is by the mechanism of improving the expression level of GH mRNA and IGF-1 mRNA. Methods The experimental points four groups as following:SAMR1 mice which feed saline lavage,SAMP6 divid as saline lavage group,subcutaneous injection of rhGH group and a kidney-tonifying herbal fufang treatment group. All intervention is one time everyday. After 3 months and 6 months intervention,we measure the BMD and the expression level of the GH mRNA and of IGF-1 mRNA. Results After 3 months intervention,the BMD of R1 group and the Kidney group were higher than the P6 blank group;but there is no difference in BMD between RhGH group and the P6 blank group. The effect of GH mRNA and IGF-1 mRNA expression levels:the R1 group,rhGH and kidney group were higher than the P6 blank group. After six months intervention,the BMD of the rhGH group and kidney group are higher than the P6 blank group. GH mRNA and IGF-1 mRNA expression levels:GH group and kidney group are higher than the P6 blank group. The expression level of GH mRNA and IGF-1 mRNA in four groups has positive correlation. After six months intervention ,we found the positive correlation between the expression level of GH mRNA and IGF-1 mRNA and each part of the whole body BMD. Conclusion A kidney-tonifying herbal fufang can improve the bone mineral density of P6,and its mechanism may be related to improve expression level of GH mRNA and IGF-1 mRNA.