乙型肝炎病毒核心蛋白区突变与隐匿性乙型肝炎病毒感染的相关性

摘要

Objective To investigate the role of mutations in C region that may contribute to occult hepatitis B virus infection.Methods C genes were amplified from two OBI blood donor samples respectively.Plasmids with mutations in C region of hepatitis B virus were constructed by overlapping PCR.HBsAg and HBeAg in Huh7 cells and in the serum of Balb/c mice were detected by CMLA.HBcAg in liver tissue was detected by immunohistochemistry,while HBV-RNA was tested by RT-PCR.Results Mutations in C region significantly reduced the expression level of HBeAg and HBcAg,but had no significant effect on HBsAg and HBV-RNA.Conclusion The mutations in C region affect the expression level of HBeAg and HBcAg,which may play an important role in the occurrence of OBI.%目的:探索乙型肝炎病毒核心蛋白区(C区)变异在隐匿性乙型肝炎病毒感染(OBI)中的作用.方法:从2例献血者OBI的样品中扩增得到C区基因构建乙型肝炎病毒C区突变型质粒,检测各突变型质粒在HuH7细胞中HBsAg和HBeAg的水平.Balb/c小鼠高压尾静脉注射突变型质粒检测其血清HBsAg和HBeAg的水平,免疫组织化学检测肝组织HBcAg的表达及分布情况,RT-PCR检测肝组织HBV-RNA的水平.结果:与野生型相比,C区突变型显著性下调HBeAg和HBcAg的表达水平,对HBsAg表达水平和HBV-RNA的水平无显著性作用.结论:OBI患者体内的乙肝病毒C区变异改变HBV病毒的HBeAg和HBcAg的表达水平,对OBI的形成具有重要作用.