Determination of phthalate esters in physiological saline solution by monolithic silica spin column extraction method

摘要

Monolithic silica spin column extraction(MonoSpin-SPE)was developed as a simple,sensitive,and eco-friendly pretreatment method which combined with ultra-fast liquid chromatography-mass spectrometry(UFLC-MS)to determine the levels of six phthalate esters,dimethyl-(DMP),diethyl-(DEP),dipropyl-[DPrP],butyl-benzyl-(BBP),dicyclohexyl-(DcHP),and di-n-octyl-(DOP)phthalate in physiological saline samples.Under optimized experimental conditions,the method was linear in the following ranges:0.2-50 μg/L for DMP,DEP,DPrP,DcHP and DOP;5-100 μg/L for BBP.The correlation coefficients(R2)were in the range of 0.9951-0.9995 for all the analytes and the limits of detection(LODs)and limits of quantification(LOQs)were in the ranges of 0.02-0.9 μg/L and 0.08-2.7 μg/L,respectively.The pretreatment process showed good reproducibility with inter-day and intra-day relative standard deviations(RSDs)below 8.5% and 11.2%,respectively.This method was used to determine the levels of six phthalate esters in physiological saline samples and the recoveries ranged from 71.2% to 107.3%.DMP and DEP were found in actual physical saline samples(brand A and brand B).