长波紫外线和中波紫外线对去卵巢骨质疏松大鼠1,25-二羟基维生素D3和骨代谢影响的比较

摘要

To investigate the effects of ultraviolet A (UVA) and ultraviolet B (UVB) on serum 1,25-dihydroxy-vitamin D3 and bone metabolism of ovariectomized rats and to compare them on bone metabolism.Methods:In the study,40 six-month female Sprague-Dawley rats were randomly divided into sham-operated control group (sham),osteoporosis model group (OVX),UVA irradiation group (OVX + UVA) and UVB irradiation group (OVX + UVB).Except the sham-operated control group,the remaining rats were ovariectomized to establish the osteoporosis models.After the model establishment,the UVA and UVB groups were exposed to UV irradiation with wavelengths of 340 nm and 313 nm,respectively.After the 15-week UV irradiation treatment,bone material density and serum 1,25 (OH)2D3,osteocalcin (bone gamma-carboxyglutamic acid protein,BGP),calcium (Ca) and phosphorus (P) contents were measured.Results:Compared with the sham group,the body weights [(486.5±55.7) g,(488.3±32.1) g,(494.1 ±49.8) g,vs.(408.6±36.1) g,P＜0.01] were significantly higher in the model group,UVA group and UVB group; proximal femur BMD [(0.318 ±0.025) g/cm2,(0.316 ± 0.031) g/cm2,(0.322 ± 0.036) g/cm2,vs.(0.386 ± 0.027) g/cm2,P ＜0.01],central BMD [(0.321 ± 0.038) g/cm2,(0.319 ± 0.051) g/cm2,(0.320 ± 0.053) g/cm2,vs.(0.347 ±0.044) g/cm2,P ＜0.05],distal femur BMD [(0.320 ±0.028) g/cm2,(0.318 ±0.030) g/cm2,(0.322 ±0.036) g/cm2,vs.(0.361 ±0.046) g/cm2,P ＜0.01] were significantly lower in the model group,UVA group and UVB group.After the 15-week treatment of UV radiation,compared with the sham group,proximal femur BMD [(0.162 ±0.125) g/cm2 vs.(0.293 ±0.076) g/cm2,P ＜ 0.01],central BMD [(0.205 ± 0.102) g/cm2 vs.(0.306 ± 0.031) g/cm2,P ＜ 0.01],distal femur BMD [(0.153 ±0.119) g/cm2 vs.(0.274 ±0.017) g/cm2,P ＜0.01] were significantly decreased in the model group ; serum 1,25 (OH) 2D3 [(19.80 ± 1.67) ng/L vs.(28.35 ± 4.32) ng/L,P＜0.01],BGP [(11.00 ±0.01) ng/L vs.(16.64 ±0.01) ng/L,P＜0.01] and Ca [(2.14 ±0.10)mmol/L vs.(2.68 ±0.16) mmol/L,P ＜0.01] were significantly lower in the model group.Compared with the model group,proximal femur BMD [(0.248 ± 0.092) g/cm2,(0.218 ± 0.123) g/cm2,vs.(0.162±0.125) g/cm2,P＜0.01],central BMD [(0.272 ±0.010) g/cm2,(0.275 ±0.036) g/cm2,vs.(0.205 ±0.102) g/cm2,P＜0.01] and distal femur BMD [(0.251 ±0.009) g/cm2 (0.242 ±0.063) g/cm2,vs.(0.153±0.119) g/cm2,P ＜0.01] were significantly increased in the UVA group and UVB group; serum 1,25(OH)2D3[(29.47±4.54) ng/L,(27.56 ±6.33) ng/L,vs.(19.80±1.67) ng/L,P＜0.01],BGP[(15.70 ± 0.01) ng/L,(15.62 ±0.02) ng/L,vs.(11.00 ±0.01) ng/L,P ＜ 0.01] and Ca [(2.48 ± 0.22) mmol/L,(2.58 ± 0.13) mmol/L,vs.(2.14 ± 0.10) mmol/L,P ＜ 0.01] were significantly higher in the UVA group and UVB group.There were no statistical differences among the UVA group,UVB group and sham group.Conclusion:Both UVA and UVB may improve serum 1,25 (OH) 2D3 content of ovariectomized rats,promote bone formation,increase bone material density,and relieve bone loss due to osteoporosis.%目的:探讨长波紫外线(ultraviolet A,UVA)和中波紫外线(ultraviolet B,UVB)对去卵巢骨质疏松(osteoporosis)模型大鼠的血清1,25-二羟基维生素D3[1,25-dihydroxy-vitamin D3,1,25(OH)2D3]和骨代谢的影响,并比较UVA和UVB的治疗效果.方法:将40只健康雌性6月龄SD大鼠随机分为假手术对照组(sham)、模型组(OVX)、UVA照射组(OVX+UVA)、UVB照射组(OVX+ UVB).除假手术对照组外,其余各组经双侧卵巢切除术建立骨质疏松大鼠模型.模型建立后,对UVA照射组和UVB照射组分别使用波长340 nm和313 nm的紫外线进行照射治疗,15周后测定大鼠骨密度及血清1,25(OH)2D3、骨钙素(bone gamma-carboxyglutamic acid protein,BGP)、钙(calcium,Ca)和磷(phosphorus,P)的含量.结果:去卵巢术后6个月,与假手术对照组相比,模型组、UVA照射组和UVB照射组大鼠体重[(486.5±55.7)g,(488.3 ±32.1)g,(494.1 ±49.8)g,vs.(408.6±36.1)g,P＜0.01]显著升高,股骨近心端骨密度[(0.318±0.025)g/cm2,(0.316±0.031) g/cm2,(0.322±0.036) g/cm2,vs.(0.386±0.027) g/cm2,P＜0.01]、中心骨密度[(0.321±0.038) g/cm2,(0.319±0.051) g/cm2,(0.320±0.053) g/cm2,vs.(0.347±0.044) g/cm2,P＜0.05)]和远心端骨密度[(0.320±0.028)g/cm2,(0.318 ±0.030) g/cm2,(0.322±0.036) g/cm2,vs.(0.361±0.046)g/cm2,P＜0.01]显著降低,差异有统计学意义.紫外线照射15周后,与假手术对照组相比,模型组大鼠股骨近心端骨密度[(0.162 ±0.125) g/cm2vs.(0.293±0.076) g/cm2,P＜0.01]、中心骨密度[(0.205±0.102)g/cm2vs.(0.306±0.031) g/cm2,P＜0.01]和远心端骨密度[(0.153±0.119) g/cm2vs.(0.274±0.017) g/cm2,P ＜0.01]显著降低,血清1,25 (OH)2 D3[(19.80±1.67)ng/L vs.(28.35±4.32) ng/L,P＜0.01]、骨钙素[(11.00±0.01) ng/L vs.(16.64±0.01) ng/L,P＜0.01]和钙[(2.14±0.10) mmol/L vs.(2.68±0.16)mmol/L,P＜0.01]含量显著降低,差异有统计学意义.与模型组相比,UVA照射组和UVB照射组股骨近心端骨密度[(0.248±0.092) g/cm2,(0.218±0.123) g/cm2,vs.(0.162±0.125) g/cm2,P＜0.01]、中心骨密度[(0.272±0.010) g/cm2,(0.275±0.036) g/cm2,vs.(0.205±0.102) g/cm2,P＜0.01]、远心端骨密度[(0.251±0.009) g/cm2,(0.242±0.063) g/cm2,vs.(0.153±0.119) g/cm2,P＜0.01]显著升高,血清1,25(OH)2D3[(29.47±4.54) ng/L,(27.56±6.33)ng/L,vs.(19.80±1.67)ng/L,P＜0.01]、骨钙素[(15.70±0.01) ng/L,(15.62±0.02) ng/L,vs.(11.00±0.01) ng/L,P ＜0.01]、钙[(2.48±0.22) mmol/L,(2.58±0.13) mmol/L,vs.(2.14±0.10) mmol/L,P＜0.01]含量显著升高,差异有统计学意义.与UVB照射组相比,UVA照射组大鼠血清1,25(OH)2D3、骨钙素、钙和磷含量及骨密度的差异无统计学意义.结论:UVA和UVB均能提高去卵巢骨质疏松模型大鼠血清1,25(OH)2D3含量,促进骨形成,升高骨密度,缓解骨质疏松造成的骨质丢失.