Con A型差异糖蛋白在大鼠肝再生中的作用研究

摘要

To explore the role of glycoprotein in rat liver regeneration,this study analyzes the glycoprotein differential expression profile during rat liver regeneration using Concanavalin-A affinity chromatography,2D-PAGE and MALDI-TOF/TOF technology.The result shows that,among 123 proteins,there are 117 proteins which has potential N-or O-glycosylation site.Difference analysis indicates that,33 proteins have differential expression during liver regeneration,the numbers of up-,down-and up/down-regulated proteins are 20,9 and 4,respectively.Pathway analysis by IPA software shows that,these differential proteins mainly participate in p70S6K and PI3K/AKT signaling pathway which maybe regulate liver regeneration.Further analysis reveals that,the protein of NR1H4,GNMT,F16P,BGLR,H6PD,ALDH,14-3-3Z,ASSY,CYTB and ACTB are closely related to rat liver regeneration after partial hepatectomy.This research will contribute to study the glycopeptideˊs structure of glycoprotein and the bio-function of protein glycosylation,especially the role in liver regeneration.%为研究差异糖蛋白在肝再生中的作用,运用Con A凝集素固相亲和层析、双向电泳和质谱等技术对大鼠再生肝的糖蛋白质组学进行研究,共鉴定出123种蛋白.有117种蛋白具有潜在的N-或O-糖基化位点；差异分析结果表明,共有33种糖蛋白在肝再生中发生有意义表达,其中,有20种蛋白发生上调表达,9种蛋白发生下调表达,4种蛋白发生上/下调表达.IPA分析表明这些差异蛋白主要参与p70S6K信号通路、PI3K/AKT通路,进一步分析发现NR1 H4、GNMT、F16P、BGLR、H6PD、ALDH、14-3-3Z、ASSY、CYTB和ACTB与大鼠肝再生相关,这些糖蛋白均含有不同程度潜在的糖基化位点.本研究有助于进一步开展糖链结构解析和糖基化对蛋白功能的影响,以及糖基化蛋白在肝再生中的作用机理研究.