小黑杨CYCD3;3基因启动子序列分析及其在拟南芥中的表达

摘要

Through the relevant website and the bioinformatics software of Poptr:CYCD3;3 promoter sequences are analyzed in detail , we cloned 2000 bp fragment from Populus DNA, and constructed the plant expression vector ( pCAMBIA1301-Pro-moter:CYCD3;3) and floral dip transformation method using wild-type Arabidopsis thaliana.From the resistance screening and molecular detection results , PCAMBIA1301-Promoter:CYCD3;3 gene was integrated into the genome of Arabidopsis thaliana.By the transgenic seedlings Gus staining observation , the gene expression patterns were mainly concentrated in the plant meristem of expression .Therefore, the Poptr:CYCD3;3 regulatory CYCD3;3 gene in cell division would play an important role .%通过相关网站及生物信息软件对Poptr:CYCD3;3基因启动子序列进行详细分析,从小黑杨(Popu-lus)叶片总DNA中克隆到2000 bp的目的片段,构建植物表达载体(pCAMBIA1301-Promoter:CYCD3;3),并利用浸花法转化野生型拟南芥(Arabidopsis thaliana);通过抗性筛选和分子检测结果显示,pCAMBIA1301-Promoter:CY-CD3;3基因已经整合到拟南芥基因组中.同时对获得的转基因幼苗进行GUS染色并观察,其基因表达模式主要集中在植物分生组织中,由此表明Poptr:CYCD3;3调控CYCD3;3基因在细胞分裂中发挥作用.