毛果杨 PtAREB1基因启动子的克隆及功能

摘要

为了研究杨树ABF2同源基因的表达规律，从毛果杨基因组DNA中克隆出PtAREB1基因上游一段1800 bp序列。序列分析结果表明，该序列含有逆境胁迫响应元件TC－rich repeats、ABA应答元件ABRE和茉莉酸甲酯（ Methyl Jasmonate ，MeJA）应答元件TGACG－motif等胁迫相关元件。在序列分析的基础上，构建了PtAREB1基因启动子驱动GUS 报告基因的植物表达载体，利用农杆菌介导的花粉管通道法获得转基因拟南芥。结果表明PtAREB1启动子可以在干旱、ABA、盐、MeJA和SA胁迫下，驱动GUS基因在转基因拟南芥的根、茎和叶中表达。说明PtAREB1基因可能与干旱、高盐等胁迫应答紧密相关。%In order to study the expression and regulation of ABF homologous gene in poplar , a 1 800 bp 5’ flanking sequence of PtAREB1gene was isolated by PCR from genomic DNA of Populus trichocarpa.By promoter sequence analysis , the se-quence contained stress-response element TC-rich, ABA-response element ABRE and MeJA-response element TGACG-motif.By sequence analysis , the PtA REB1promoter was fused to the GUS reporter gene to characterize its expression pat -tern in P.trichocarpa.From the Agrobacteir um mediated transformation of Arabidopsis th aliana, the GUS gene was induced in Arabidopsis thaliana, and it expressed in roots, stems and leaves under ABA, drought, high salt, MeJA and SA, sug-gesting the PtAREB1 promoter was responsive to ABA , drought and high salt stress .