酯交换植物油中甾醇测定方法研究

摘要

采用国标铁矾显色法(GB/T 5009.128-2003)测定酯交换植物油中甾醇的含量,通过与改进的Lieberman-Burchard比色法和毛地黄皂苷测定结果进行对比分析,确定了最佳显色剂用量、显色剂组成比例及显色时间.研究结果表明国标铁矾显色法最佳显色条件为:显色剂用量2 mL,显色剂组成比例1:9,显色时间15min.因此,采用国标铁矾显色法测定酯交换植物油中高含量甾醇的平均误差为4.54%,平均回收率为98.61%.可见该方法可为添加甾醇功能性植物油的研究提供参考,并可作为产业化生产的一种快捷、简便的监测手段.%The national standard Iron-Alum method (GB/T 5009.128-2003) was studied for etermination of sterol content in the transesterificational vegetable oil, and the optimum coloring conditions were confirmed through analyzed the results and compared with the improved Lieberman-Burchard colorimetric method and digitonin method. The results showed that the optimum coloring conditions of the national standard Iron-Alum method were as following: coloring reagent volume was 2 Ml, coloring reagent ratio was 1:9, and coloring time was 15 min. Therefore, the average relative error and reproducibility is 4.54% and 98.61% by using the national standard Iron-Alum method for determination of high content of sterol in the transesterificational vegetable oil. The method can be used as a quick and simple supervise method for the study of functional oil appended with sterol and industrial product.