Petals of Gladiolus 'Rose Supreme' were used to establish regeneration system.Developmental characteristics of petals were observed.A total of 94 plantlets of petal somaclonal line were marked in M1 generation by ISSR molecular markers and traits were observed in M3 generation.The results showed that the best medium to induce callus was MS+2,4-D 4.0 mg · L-1+6-BA 0.5 mg · L-1 and the best medium to induce somatic embryogenesis was MS+2,4-D1.0 mg · L-1+TDZ0.3 mg · L-1.New petals could be formed from petal callus directly.Pigments of mother plant appeared on plantlets while they were formed from petals.Two mutants were obtained from petal somaclonal line in M3 generation which demonstrated the correctness of ISSR analysis in M1 generation.
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