Effects of Matrine on Bovine Papilloma Virus-infected C127 Cells

摘要

The aim of this study was to select drugs for prevention and treatment of bovine papilloma in vitro, using bovine papilloma virus(BPV-2) infected mouse mammary epithelial(C127) cell lines as the infectious cell model system. C127 cell shapes changed with BPV infection observed under light microscopy. C127 cell shapes changed with BPV infection observed under light microscopy. PCR was used to confirm BPV infected C127 cells. mR NA levels of matrine to BPV-2 infected C127 cells were detected by RT-PCR. Apoptosis of BPV-infected C127 cells treated with matrine was analyzed by flow cytometry. Results showed that 80 μg· mL^(-1) matrine had a direct virucidal and intervention effect of BPV-2. Matrine inhibited the proliferation of BPV-infected cells. Therefore, matrine might be regarded as a therapeutic drug candidate for BPV induced bovine papilloma disease treatment in future.