介入栓塞法小型猪急性心肌梗死模型的建立

摘要

Objective:To establish acute myocardial infarction models by interventional embolization in mini-pigs. Methods:E-leven mini-pigs,weighting 30-45kg,were anaesthetized. Echocardiography and electrocardiogram examination as well as coronary artery digital subtraction angiography ( DSA) were performed,and myocardial enzymes and troponin were tested before embolization. The distal of left descending artery or left circumflex artery was embolized with sterilized thread segment. In the 11 pigs,embolization was followed by the DSA procedure and electrocardiogram examination. Myocardial enzymes and troponin were tested and echocardiography was per-formed 24 hours after embolization,and pathologic histology was examined 5 weeks after embolization. Paired t-tests were used in the in-dex of laboratory test and heart function before and after the operation. Results:The model of myocardial infarction was successfully es-tablished in 8 of 11 mini-pigs. The successful rate was 72%. All of the results were normal before embolization. After embolization,the coronary artery DSA showed that the object vessels were obstructed. The electrocardiogram examination showed abnormal changes in dif-ferent degrees. The value of myocardial enzymes,troponin,ejection fraction and fractional shortening were significantly different between preoperative and postoperative examination (P<0. 05). The infarction sites were confirmed by pathologic histology after the operation. Conclusion:The technique of interventional embolization is simple and accessible,and has high successful rate. It is one of the best methods to establish stable myocardial infarction models.%目的：：用介入栓塞法建立小型猪的急性心肌梗死模型。方法：11头贵州种小型猪，体重30~45 kg。术前采集血液进行实验室检查(心肌酶谱和肌钙蛋白等)；并行心电图及超声心动图检查。常规冠状动脉造影后，经导管将栓子推送到左冠状动脉前降支或回旋支远端进行栓塞，复查造影结果满意后结束手术操作，并复查心电图。术后24 h采集血液复查实验室指标，并复查超声心动图。术后5周处死实验动物行心脏病理组织学检查。手术前后的实验室检查指标及心功能指标(射血分数和短轴缩短率)采用配对t检验。结果：11头小型猪中，有8头成功建立急性心肌梗死模型，建模成功率72%。栓塞前：各项检查结果正常。栓塞后：冠状动脉造影检查栓塞远端未见显影；心电图出现不同程度的异常改变；实验室检查及心功能检查与术前比较，其差异具有统计学意义(P<0．05)；术后病理证实均有梗塞灶的存在。结论：介入栓塞法成功率高，简单易行，是建立稳定心肌梗死模型的较好方式之一。