miR-223基因家族的分子进化与靶基因预测

摘要

Objective:This study aims to investigate the function of miR-223 gene family members,predict the miR-223 target gene and analyze the biological function of the miR-223 target gene.Methods:The sequence characteristic and molecular evolution of miR-223 were analyzed by Clustal X 1 .83 and MEGA 5.0.TargetScan,PicTar and miRDB were used to predict target genes and the GO and KEGG were used to analyze the function annotation.Results:27 members of miR-223 gene family were obtained from miRBase database.Most of them were located in the intergenic region and a few in unknown region.A majority of miR-223 existed in X chromo-some and a few in autosome.miR-223 mature sequences were from 20 to 23 nt in length and possessed high homology.Phylogenetic a-nalysis indicated three sublines of miR-223 gene family members.27 target genes of miR-223 were predicted.And they were related with the process of signal transduction,regulation of transcription,cell growth and development and so on.Conclusion:Those results are not only of potential importance for further study on the function of miR-223 gene family members,but aslo for providing theory basis of subsequent experiment research.%目的：分析所有 miR-223基因家族成员的序列特征，并对 miR-223靶基因进行预测，然后分析 miR-223靶基因的生物学功能。方法：利用 Clustal X 1．83软件分析 miR-223基 因 序 列 特 征，MEGA 5．0软件分析进化关系，再运用 Tar-getScan、PicTar 和 miRDB 进行靶基因预测，最后 GO 和 KEGG 进行功能分析。结果：在 miRBase 数据中获得 miRNA-223基因家族成员的序列27条，绝大部分位于基因间隔区，少数成员的基因位置未知。大部分定位于 X 染色体，部分成员位于常染色体。miRNA-223成熟序列长度在20-23nt 之间，同源性较高。系统进化树分析表明，miRNA-223基因家族成员分为三支。预测获得人类 miRNA-223的可能具有27个靶基因，它们主要与信号转导、转录调控以及细胞生长发育等密切相关。结论：本研究结果不仅有利于理解 miR-223基因家族的生物学功能，也可为后续研究提供理论依据。