Objective: To analyze the silver sulfadiazine and mupirocin, clotrimazole alone or in combination with antibacterial activity study of MRSA. Methods: A retrospective analysis of our hospital in March 2014 to 2015 March treated 100 cases of burn patients medical records, made of burn wound secretion samples were separated and 100 strains of non repeated and continuous MRSA, use checkerboard method of sulfadiazine silver and mupirocin, clotrimazole alone and combined with the MRSA antibacterial activity. Results: A MIC of the MRSA was 8g/mL, and the MIC50 was 9g/mLL [the same as the drug concentration in different MIC. ], MIC90 16μg/mL, mupirocin, Luoxing against MRSA a mic 2μg/mL, the MIC50 16g/mL; MIC90 for 64μg/mL, clotrimazole against MRSA a mic were 2μg/mL, MIC50 3μg/mL, MIC90 for 4μg/mL [ibid.]. Combined use of mupirocin and clotrimazole, silver and sulfadiazine in comparison, MIC50 reduced by 75%, MIC90 reduce 87.5%;and mupirocin, MIC50 reduce 87.5%, MIC90 reduced 96.9%;and clotrimazole in comparison,MIC50 is reduced by 50%, MIC90 reduce 50.0%. Conclusion:The MRSA infection of burn wound, using the low dosage of silver sulfadiazine in combination with other drugs, not only can realize the effective control of infection, but also can reduce a drug on wound healing of adverse reaction. It is worthy of clinical application.%目的：研究分析三种局部抗菌药物对甲氧西林耐药金黄色葡萄球菌的抗菌活性研究。方法：回顾性分析我院于2014年3月～2015年3月收治的100例烧伤患者的病历资料，对烧伤创面分泌物制作标本，共分离100株非重复并连续的MRSA，应用棋盘格法对磺胺嘧啶银、莫匹罗星、克霉唑单用以及联用时对MRSA的抗菌活性予以分析。结果：磺胺嘧啶银对MRSA的MIC为8μg/mL， MIC50为9μg/mL，MIC90为16μg/mL，莫匹罗星对MRSA的MIC为2μg/mL，MIC50为16μg/mL，MIC90为64μg/mL，克霉唑对MRSA的MIC分别为2μg/mL，MIC50为3μg/mL，MIC90为4μg/mL。莫匹罗星和克霉唑联用，和磺胺嘧啶银单用比较，MIC50降低75%，MIC90降低87.5%；和莫匹罗星单用比较，MIC50降低87.5%，MIC90降低96.9%；和克霉唑单用比较，MIC50降低50%，MIC90降低50.0%；磺胺嘧啶银和莫匹罗星联用，有70例相同，20例相加，10例无关，没有拮抗现象。磺胺嘧啶银和克霉唑联用，92例抗菌效果相加，8例无关，没有协同和拮抗现象。两种药物联用的抗菌活性明显优于单用。结论：MRSA感染的烧伤创面，就要应用较低剂量的磺胺嘧啶银联合其他药物，不仅可以实现感染的有效控制，也能将药物对创面愈合的一种不良反应降低，值得临床推广。
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