HPLC法测定复方首乌地黄丸中二苯乙烯苷的含量

摘要

Objective:To establish method for determining the content of 2,3,5,4'-tetrahydroxy stilbene-2-O-β-D- glycoside(simply named as 2,3,5,4'-stilbene glycoside)from Radix Polygoni Multiflori Prepareparata in Shouwu Dihuang Compound Pill by HPLC. Methods:It was performed by HPLC with WIech UItimate R XB-C18 (250mm×4.6mm,5μm). The mobile phasae consisted of acetonitrile and water(18∶82).The flow rate was 1.0mL·min-1 with the detection wavelength at 320nm. Results:2,3,5,4'-stilbene glycoside showed a good linear relationship within the range of 0.052~1.05μg (R=0.9999). The average recovery was 99.42% with RSD=0.52%,(n=6). Conclusion:The method is simple,rapid, accurate and reliable,and it can be applied to control the quality of Shouwu Dihuang Compound Pill.%目的：建立高效液相色谱法测定复方首乌地黄丸中制何首乌的2，3，5，4'-四羟基二苯乙烯-2-0-/3-D-葡萄糖苷（简称二苯乙烯苷）的含量。方法：采用高效液相色谱法，色谱柱为WIech UItimate R XB－C18（250mm×4.6mm，5μm），流动相为乙腈-水（18∶82），检测波长为320nm，流速为1.0mL·min－1。结果：二苯乙烯苷在0.052～1.05μg范围内线性关系良好（R=0.9999）。平均回收率为99.42%（RSD=0.52%n=6）。结论：此方法简单，快速，重现性好，可作为复方首乌地黄丸的质量控制方法。