VEGF和ERK1/2在不同周龄自发性高血压大鼠肾脏中的表达

摘要

目的 研究血管内皮生长因子(VEGF)和细胞外信号调节激酶(ERK1/2)在自发性高血压大鼠(Sponta Neously Hypertensive Rats,SHR)不同周龄组肾脏中的表达,探讨它们在高血压肾病发生发展中的作用.方法 选取4个不同周龄组(4、8、16、20周龄)SHR和正常血压大鼠,将肾脏作常规病理和免疫组化染色,观察肾脏的形态学改变并分析VEGF和ERK1/2在肾脏中的表达.结果 SHR从8周龄开始血压升高,随着血压的不断升高,SHR肾小球中VEGF的阳性表达也有所增加,16、20周龄组SHR的表达高于4、8周龄组(P＜0.05).ERK1/2在16、20周龄SHR的肾脏细动脉、小叶间动脉、叶间动脉和弓形动脉中的内皮细胞和血管平滑肌细胞中均有阳性表达,而且阳性细胞数明显多于4周龄SHR(P＜0.05).结论 ERK1/2、VEGF可能参与高血压肾脏病变的发生发展过程.%Objective To investigate the expression of vascular endothelial growth factor (VEGF) and extracellular signal -regulated kinase l/2(ERKl/2) in kindeny of spontaneously hypertensive rats(SHR) at different weeks of ages,and to elucidate the role of VEGF and ERK1/2 in occurrence and development of hypertension nephropathy. Methods The spontaneous hypertensive rats at different weeks of ages (4 weeks, 8 weeks, 16 weeks, 20 weeks) and normal blood pressure rats were selected. The renal morphological changes were observed and the expression of VEGF and ERK1/2 in kidney was analyzed using conventional pathology and the immunohistochemical S - P method. Results The blood pressure elevated from 8 - week old in SHR. Along with blood pressure elevation, the VEGF positive expression increased in the glomeruli of SHR, the expression of 16W - SHR, and 20W - SHR at 16, 20 weeks of ages was significantly higher than those at 4, 8 -week old. The positive expression of ERK1/2 were found in endothelial cells and vascular smooth muscle cells of renal afferent arterioles, interlobular arteries, interlobar arteries and arcuate artery in 16WSHR, 20 WSHR, and the number of positive cells were higher than that of 4WSHR (P < 0. 05). Conclusion It is extrapolated that ERK1/2 and VEGF participate in the occurrence and development process of hypertension possibly nephropathy.