P-选择素在基因敲除小鼠肺转移中的作用研究

摘要

Objective To study the effect of P-selectin on pulmonary metastasis of melanoma in mice. Methods B16 cells were intravenously injected into P-selectin knockout and C57/BL(C57)mice. Af-ter three weeks,the mice were sacrificed by cervical dislocation. Lung metastasis was observed by gross anato-my. HE staining of the metastatic tumor tissue sections was performed to confirm homology with B16 cells. The number of metastatic foci was counted. Results P-selectin knockout mice developed fewer metastatic foci on the lung surface than C57 mice(P ! 0.01). HE staining of the metastatic tumors confirmed their homology with B16 cells. Conclusion P-selectin loss may be involved in the inhibition of melanoma metastasis to the lung.%目的：探讨P-选择素对小鼠黑色素瘤肺转移的影响。方法将体外培养的B16黑色素细胞株通过小鼠的尾静脉注射到小鼠背景为C57/BL （C57）的P-选择素敲除的小鼠和C57小鼠的体内，建立转移瘤瘤模型，第21天断颈处死小鼠，计数转移到肺表面的结节的数目，HE染色进一步确定转移结节。结果 P-选择素敲除小鼠肺表面转移的肿瘤结节的数目明显比C57小鼠少，两者比较差异有统计学意义（P"0.01）。HE染色证实小鼠肺组织中有B16细胞的肺转移灶。结论 P-选择素缺失可能参与抑制了黑色素瘤的肺转移。