甜菜BvM14-MADSbox基因启动子的组织特异性表达

摘要

MADS - box基因在植物的花器官和各种营养器官中均有不同形式的时空表达模式,行使不同功能.实验室前期获得了甜菜M14品系BvM14 - MADS box基因的特异启动子Pbm,并对其进行了瞬时表达.本试验利用含有重组载体pBI121 - Pbm - GUS的农杆菌转化烟草,对报告基因GUS在转基因烟草各组织表达特性进行研究,探讨BvM14 - MADS box基因特异启动子Pbm是否具有组织特异性表达活性.试验结果表明,GUS基因只在花的萼片中有表达,在根、茎、叶中均无表达,可见此启动子Pbm在花组织的萼片中具有表达特异性.%The members of MADS-box family have different spatial and temporal expression patterns in flower meri-stem, floral organs and a variety of vegetative organs, and play different functions. In previous study, the BvMlA-MADS box promoter Pbm was cloned and made it transient expression, but didn' t verified its expression pattern in different tissues of the transgenic tobacco. The tobacco with the engineeed Agrobacterium tumefaciens EHA105 was transformed that contained the recombinant plasmid pBI121-Pbm-GUS with the promoter Pbm replaced the 35 S promoter. Followed selecting the positive plants, the expression of GUS in different tissues of transgenic tobacco was tested. The result showed that the GUS gene only can be expressed in sepal, but not in leaf, stem and root, indicating that the BvMl4-MADS box promoter Pbm had a special activity in floral organ.