c-MYC蛋白在肝细胞癌组织中的表达及临床病理意义

摘要

Objective: To investigate the expression of c-MYC protein in hepatocellular carcinoma(HCC) and paracance-rous liver tissues of patients and to analyze its correlation with clinicopathological features. Methods: 158 samples of HCC, corresponding adjacent liver tissues from Affiliated Nantong Third Hospital of Nantong University between Jan. 2005 and Dec. 2011 were collected. Immunohistochemical staining was performed to detect the c-MYC protein expression in HCC tissues and corresponding adjacent liver tissues. The correlation between c-MYC protein expression and clinicopathological features of HCC was analyzed. Results: Immunohistochemical staining showed c-MYC protein expression demonstrate cellular nucleus reactivity in HCC cells but no positive staining in paracancerous liver cells. The positive rate of c-MYC protein expression in HCC tissues was 26.58%(42/158). The c-MYC expression was correlated with liver cirrhosis( r=-0.174, P=0.028), differentiation degree of HCC(r=0.182, P=0.021) and relapse(r=0.282, P=0.001), but not correlated with age, gender, the level of serum alpha fetal protein, the hepatitis B virus, diameter of tumor, tumor number and vascular invasion. Conclusion:High c-MYC expression in the HCC may be related with the ability of growth and invade of tumor cells.%目的：检测肝细胞癌(hepatocellular carcinoma, HCC)患者癌组织中c-MYC蛋白的表达情况，并分析其临床病理意义。方法：收集南通大学附属南通第三医院2005年1月—2011年12月158例HCC手术标本，运用免疫组织化学染色法检测c-MYC蛋白在癌及癌旁组织中的表达情况，结合临床病理资料进行统计分析。结果：c-MYC蛋白在癌旁组织中不表达；在HCC组织中阳性信号定位于细胞核；c-MYC蛋白在HCC组织中阳性表达率为26.58%(42/158)，c-MYC蛋白在HCC组织中表达高于癌旁组织(χ2=48.438, P=0.000)；c-MYC蛋白在HCC组织中的表达与有无肝硬化(r=-0.174, P=0.028)、病理学分级(r=0.182, P=0.021)和术后复发(r=0.282, P=0.001)相关，而与HCC患者年龄、性别、HBsAg感染、血清甲胎蛋白水平、肿瘤直径、肿瘤个数及血管侵犯均未见明显相关。结论：c-MYC蛋白在HCC高表达可能与HCC的发生、生长和侵袭相关，未来可能用于HCC的恶性程度评估或预后判断。