ABSTRACT:OBJECTIVE To study the related basic research of high salt-induced kidney injury in SHR rats,and discuss the salt-hurt-blood connotation of traditional Chinese medicine.METHODS WKY rats were set as the normal group.SHR were randomly divided into 2 groups:normal group(SHR)and high-salt feed group(SHR+HS).SHR+HS group rats were given 4% high salt diet from the 10th week.Urine trace albumin and NAG in urine were detected every two weeks.On the 20th week,the hemorheology indexes and coagulation four indices were measured.The rat kidney was divided into two parts.One was fixed with formalin and detected with HE;the other part was used to detect ICAM-1,VCAM-1 and IL-6 expressions.RE-SULTS After feeding high-salt feed for 6 weeks,urine trace albumin and NAG increased significantly(P <0.05).On the 20th week,the whole blood viscosity and blood plasma viscosity in SHR+HS group were significantly(P <0.05)greater than SHR group.APTT and PT were significantly reduced and FIB significantly increased(P <0.05).Renal pathology index in SHR+HS group was higher than SHR group,and the kidney inflammation factors ICAM 1,VCAM 1 and IL-6 increased(P <0.05). CONCLUSION High salt can increase the whole blood viscosity,disorder APTT,PT and FIB,increase the expression of ICAM 1,VCAM 1 and IL-6,and strengthen the kidney injury of SHR rats,which might partly explain the scientific connota-tion of"salt-hurt-blood" and"salty flavor entering the kidney".%目的：研究关于高盐诱导的 SHR 大鼠肾损伤的相关基础,并探讨中医理论“盐胜血”的内涵。方法 WKY 大鼠为正常组,SHR 大鼠随机等分为2组,分为普通饲料组(SHR)与高盐饲料组(SHR+HS),SHR+HS 组大鼠从10周龄开始给予4%高盐饮食。每2周检测尿 m-ALB 与 NAG。20周龄大鼠麻醉后腹主动脉取血,检测血液流变学指标与凝血4项；处死大鼠,取肾脏,分为2部分,一部分中性福尔马林固定,HE 病理检测；一部分采用 Western blot 法检测细胞间粘附因子1(ICAM-1)、血管细胞粘附因子1(VCAM-1)、白介素6(IL-6)的表达情况。结果喂养高盐饲料6周后,尿 m-ALB 与 NAG 显著增高(P<0.05)。20周龄时 SHR+HS 组的全血黏度值与血浆黏度值显著大于 SHR 组(P <0.05)；APTT、PT 显著缩短(P <0.05)及FIB 显著增加(P <0.05)；肾脏病理检测可见 SHR+HS 组的病变积分值更高,且肾脏炎症因子 ICAM-1、VCAM-1、IL-6表达增加(P <0.05)。结论高盐可以增加 SHR 组大鼠的全血黏度、缩短 APTT、PT 与增加 FIB,增加肾脏炎症因子 ICAM-1、VCAM-1、IL-6表达,加重 SHR 组大鼠的肾损伤,这可能是“盐胜血”与“咸走肾”的部分科学内涵。
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