基于代谢组学方法研究生半夏和姜半夏对BeWo细胞的毒性机制

摘要

目的 通过气相色谱-质谱联用(GC-MS)技术分析生半夏和姜半夏干预后细胞内代谢物改变,来评价生半夏和姜半夏潜在的发育毒性.方法 采用胎盘组织来源的BeWo细胞系构建体外胎盘模型,运用GC-MS检测细胞经生半夏及姜半夏干预后细胞内所含代谢物及其相对含量的变化.结果 细胞代谢物中共鉴定出甘氨酸、氨基丙二酸、脯氨酸、葡萄糖、半乳糖、硬脂酸在、肌醇等九种差异性代谢物.结论 运用GC-MS代谢组学技术评价半夏及姜半夏的发育毒性具有可行性.%OBJECTIVE To evaluate the potential developmental toxicity of Pinellia Rhizoma(BX) and Pinelliae Rhizoma Praeparatum cum Zingibere et Alumina(JBX) by analyzing the changes of intracellular metabolites after the intervention of BX and JBX by gas chromatography-mass spectrometry (GC-MS).METHODS The BeWo cell line derived from placenta tissue was used to construct the model of the in vitro placenta, and the changes of the metabolites and their relative contents in the cells were detected by GC-MS after the intervention of BX and JBX.RESULTS The nine metabolites of glycine, alanine, proline, glucose, galactose, stearic acid, inositol and so on were identified.CONCLUSION GC-MS technology is feasible for evaluation of developmental toxicity of BX and JBX.