葡萄籽原花青素对变形链球菌的体外抗菌活性研究

摘要

目的 研究葡萄籽原花青素(Grape seed procyanidins,GSPC)对变形链球菌的体外抗菌活性,探讨其防龋潜能.方法采用微量肉汤法测定最低抑菌浓度(MIC)、最低杀菌浓度(MBC)、最低抑制生物膜浓度(MBIC);采用结晶紫半定量法测定生物膜生成量;硫酸-蒽酮法测定产糖量,pH计测定产酸量.结果葡萄籽原花青素的MIC值12.5 mg/mL;MBC值和MBIC值均为50 mg/mL;GSPC(≥12.5 mg/mL)降低变形链球菌生物膜的生成量,8倍MIC浓度(100 mg/mL)时抑制率达76%;GSPC在低于MIC浓度时仍具有抑制变形链球菌产糖、产酸能力.结论葡萄籽原花青素具有对变形链球菌的体外抗菌活性,并能抑制变形链球菌的生物膜和致龋毒力因子.%OBJECTIVE To study the antibacterial activity of grape seed procyanidins(GSPC)against streptococcus mutans in vitro and to explore its anti-caries potential.METHODS Broth micro-dilution method was used to determine minimal inhibi-tory concentration (MIC),minimal bactericidal concentration (MBC) and minimal biofilm inhibitory concentration (MBIC);crystal violet semi-quantitative method was used to determine biofilm-formation;anthrone sulfuric acid method was used to test glucan production and pH was used to test acid generation.RESULTS MIC value of GSPC was 12.5 mg/mL while MBC value and MBIC value were both 50 mg/mL.GSPC(≥12.5 mg/mL)could reduce biofilm formation,and especially when its con-tent increased to eight times of MIC content(100 mg/mL),the inhibition rate was able to achieve 76%.Also it had the ability of inhibiting the glucan production and acid generation at lower content than that of MIC.CONCLUSION GSPC shows anti-bacterial activity and inhibitory effects on biofilm-formation and cariogenic virulence factors against streptococcus mutans in vitro.