目的 观察中国南极考察队越冬队员外周血淋巴细胞钟基因Clock和Bmal 1表达昼夜节律性变化.方法 在中国第25次南极考察队越冬队员中选择8名队员,平均年龄38岁,均为男性,在1个昼夜周期内设立6个时点(ZT):02:00、06:00、10:00、14:00、18:00和22:00,每一时点采集外周静脉血6 mL,采集赴南极前后2组血样.用实时定量RT-PCR方法,测定不同昼夜时点(ZT)样品中核心钟基因Clock和Bmal 1的mRNA表达量,通过余弦法和Clock Lab软件获取节律参数,进行赴南极前后对比.结果 赴南极前,8个样本Clock和Bmal的mRNA表达均有显著昼夜节律特征(P＜0.05),Clock的峰值相位位于-335.85士13.80,Bmal 1的峰值相位位于-307.12±8.17.赴南极后,仅2例Clock和3例Bmal1表达还存在显著昼夜节律变化.Clock的峰值相位移位到-42.28±5.27,Bmal 1的同峰值相位移位到-184.58±29.58.结论 南极特殊周期环境对人体生物钟基因表达的昼夜节律会产生影响.%Objective To investigate the circadian expression of core clock gene, Clock and Bmal 1 ,in the peripheral blood lymphocytes of Chinese scientific expeditioners during the Antarctic winter. Methods Peripheral blood (6 mL) were collected in 8 healthy male volunteers (mean age 38 years) from wintering team of the 25th Chinese Antarctic Research Expedition at 6 time points in the day-night cycle (02: 00,06:00,10; 00,14:00,18:00 and 22:00). In addition, blood samples were collected before and after Antarctic expedition. The expression of Clock and Bmal 1 at different Zeitgeber times (ZT) was detected by RT-PCR. The circadian parameters were obtained and analyzed by the cosine function,Clock Lab software and amplitude F test for comparison before and after Antarctic expedition. Results Before Antarctic expedition,the expression of Clock and Bmal 1 mRNA showed a significant circadian feature(P<0. 05). The Peak phase of Clock at -335. 85±13. 80. The Peak phase of Bmal 1 at -307. 12±8. 17. After Antarctica, the mRNA transcriptions of 2 expeditioners' Clock and 3 expeditioners Bmal 1 continuously sustain the significant circadian oscillation among the same 8 Chinese scientific expeditioners. The Peak phase of Clock is at -42. 28±5. 27,The Peak phase of Bmal 1 is at -184. 58±29. 58. Conclusion Antarctic environment exerts an impact on human circadian clock gene expression.
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