不同大孔树脂对粉被虫草中 N6－（2－羟乙基）腺苷的分离特性研究

摘要

为选择合适的大孔树脂分离粉被虫草中的 N6－（2－羟乙基）腺苷（HEA），通过静态吸附与解吸附试验对4种大孔树脂吸附和解吸附 HEA 的效果进行比较，并对洗脱条件进行优化。结果表明 DM－130树脂对 HEA 的吸附量最大、解吸率较高；该树脂的最佳动态吸附条件是上样量为2倍树脂床体积（BV），吸附时间为6 h；最佳动态洗脱条件是依次用3 BV 蒸馏水，1 BV 5％乙醇和3.5 BV 10％乙醇冲洗柱床。在该条件下，经 HPLC 分析测定，洗脱液中 HEA 的峰面积百分比达到89.15％。%In order to select the appropriate macroporous resin to isolate N6 -(2-hydroxyethyl)-Adenosine from Cordyceps pruinosa Petch,tests using four kinds of macroporous resin to absorb and desorb HEA were a-dopted,and the elution conditions were also optimized.The results showed that DM-1 30 resin had the high-est resolution and greater adsorption for HEA.The best elution conditions for dynamic adsorption were as fol-lows:the loading amount was 2 times bed volume (BV)and the adsorption time was 6 h;the best elution conditions were that DM-1 30 resin should be eluted sequentially with 3 BV water,1 BV 5% ethanol and 3.5 BV 1 0% ethanol.HPLC analysis showed that under the optimum conditions peak area percentage of HEA was 89.1 5% in eluent.