大鼠视神经夹持伤后视网膜Flt-1的表达及意义

摘要

Objective To observe expression and distribution of Flt -1 in retinal on optic nerve clamping injury of rats .Methods The 12 healthy adult SD rats were surgeried that optic nerve were clamped 10s on right eye,only to be exposed optic nerve on left eye .Ex-pression and distribution of Flt -1 were deteced with immunohistochemical staining in retinal on 6h, 3d, 7d, 21d optic nerve clamping in-jury of rats.And gray value of these results were analyzed with image analyzer .Results There was a low level expression of Flt 1 protein mainly in the retina inner ,outer nuclear layer and ganglion cell layer in normal rats .After clamping the optic nerve injury ,Flt-1 began to rise at 6h, to be on top at 3d,to begin to fall at 7d,and to be lower at 21d than normal in retina .There was statistically significant of gray values in Flt-1 protein expression after injury between both eyes .Conclusion Optic nerve injury was accompanied by abnormal retinal tissue Flt-1 protein expression in rats , which could be involved in the repair of retinal and optic nerve after injury .%目的 观察大鼠视神经夹持伤后视网膜血管内皮生长因子受体-1( Flt-1)蛋白的表达变化及分布. 方法 将12只成年健康SD大鼠右眼行手术视神经夹持10s,左眼行手术只暴露视神经,不夹持. 伤后6h、3、7、21天各取3只行免疫组化染色观察视网膜Flt-1的表达变化及分布特征. 并用图像分析仪对上述结果进行灰度量化后统计分析. 结果 正常大鼠视网膜Flt-1蛋白呈低度表达,在视网膜内外核层、节细胞层均有少量表达. 视神经夹持伤后6h视网膜的Flt-1蛋白表达开始升高,3天达到峰值,7天后开始下降,21天后降至正常以下. 伤后各时间点Flt-1蛋白表达灰度值与对侧眼比较,差异均有统计学意义. 结论 大鼠视神经损伤后伴随有视网膜组织Flt-1蛋白表达的异常,后者可能参与伤后的视网膜及视神经组织的修复.