目的：近年来男性精子数量、质量呈下降趋势。文中研究小鼠膳食中高n-6-3多不饱和脂肪酸比值对其精子浓度及活度的影响。方法健康的 B6小鼠20只，随机数表法分成对照组（基础饲料）和实验组（基础饲料＋10％花生四烯酸），每组10只。喂食8周后，取各组小鼠附睾尾检测小鼠精子浓度及活度的变化，观察小鼠附睾组织显微结构的改变。结果实验组小鼠血清中α-亚麻酸、二十碳五烯酸、二十二碳六烯酸、二十二碳五烯酸、亚油酸含量均较对照组明显下降（P＜0．01），而花生四烯酸和n-6-3 PUFAs明显升高（P＜0．01）。实验组精子浓度（7×106个/mL）较对照组（80×106个/mL）显著下降，实验组精子活度（27％）较对照组（86％）显著下降，差异有统计学意义（ P＜0．01）。附睾组织管腔中精子数目明显减少。结论膳食中高n-6-3多不饱和脂肪酸比值对精子数量、质量具有显著损伤作用。%Objective Sperm quality can be affected by many factors .The aim of this study was to observe the effects of di-etary n-6-3 polyunsaturated fatty acids ( PUFAs) on sperm concentration and motility . Methods Twenty healthy B6 male mice were equally randomized to a control and an experimental group to be fed with standard diet and standard diet +10%arachidonic acid (AA), respectively, both for 8 weeks.Then the testes and epididymal tails of the mice were collected for observation of the changes in the microstructure of the epididymal tissue and determination of sperm motility and concentration . Results Compared with the con-trol group, the experimental animals showed significantly decreased serum levels of α-linolenic acid ( ALA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), docosapentaenoic acid (DPA), and linoleic acid (LA) (P <0.01), but dramatically increased the levels of AA and n-6-3 PUFAs (P<0.01).The latter also exhibited remarkable reduction in sperm concentration (7 ×106/mL vs 80 ×106/mL, P<0.01), sperm motility (27%vs 86%, P<0.01). Conclusion High levels of dietary n-6-3 PUFAs ratios reduce sperm concentration and motility in mice .
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