小鼠睾丸组织中雄激素及其受体信号抑制精母细胞干扰素刺激因子15基因表达

摘要

Objective The expression of interferon-stimulated gene 15(Isg15)was found to increase in the testes of Sertoli cell specific Ar knockout(S-Ar-/y)mice.This study aimed to exam-ine the effect of androgen and its receptor on the expression of Isg15.Methods RT-Qpcr and Western blotting were used to detect the expression of Isg15 in wide-type mice and S-Ar-/ymice and the difference was compared.The distribution of Isg15 protein in mouse testis was measured by im-munohistochemistry and immunofluorescence.Results The expression of Isg15 gene was signifi-cantly higher in S-Ar-/ymice than that in wild-type mice,and the Isg15 protein was mainly expressed in spermatocytes.Conclusion Sertoli cell specific knockout of Ar gene Results in abnormally high expression of Isg15 gene in spermatocytes.%目的 在前期研究中进行全基因组表达谱检测发现在雄激素受体(androgen receptor, Ar)基因睾丸支持细胞特异性敲除小鼠(S-Ar-/y)睾丸组织中干扰素刺激因子15(interferon-stimulated gene 15, Isg15)基因表达增高.研究的目的是了解睾丸组织中雄激素及其受体对Isg15基因表达的影响作用.方法 采用RT-qPCR和Western印迹等方法比较野生型小鼠和S-Ar-/y小鼠睾丸组织中Isg15基因表达量.通过免疫组织化学和荧光染色两种方法检测Isg15蛋白在睾丸组织中的分布.结果 在 S-Ar-/y小鼠睾丸组织中Isg15基因表达量比在野生型小鼠中显著升高,Isg15蛋白主要表达于精母细胞.结论 睾丸支持细胞特异性敲除Ar基因使精母细胞中Isg15基因表达异常增高.