探究2型单纯疱疹病毒（HSV-2）对人永生化角质形成细胞株（HaCaT）分泌白细胞介素（IL）-19、IL-20的影响

摘要

目的：：分析2型单纯疱疹病毒对人永生化角质形成细胞株分泌白细胞介素(IL)-19、IL-20的影响,为临床探讨角质形成细胞对2型单纯疱疹病毒感染初期的免疫防御机制提供依据。方法：应用2型单纯疱疹病毒感染人永生化角质形成细胞,并对感染后24、48、72h 的 IL-19、IL-20mRNA(信使 RNA)的表达量使用荧光实时定量 PCR 进行检测,分析 HSV-2对 HaCaT 细胞分泌白细胞介素(IL)-19、IL-20的影响。结果：实验组 HSV-2对 HaCaT 细胞分泌 IL-19、IL-20mRNA 表达量显著高于对照组,P <0.05。结论：角质形成细胞经2型单纯疱疹病毒感染后可分泌 IL-19、IL-20,并参与2型单纯疱疹病毒感染初期的免疫防御反应。%Objective:To analyze the influence of herpes simplex virus type 2 (HSV-2)on human immor-talized keratinocyte cells secreting interleukin (IL)-1 9 and IL-20,and to provide the basis of clinical discus-sion on immune defense mechanism of keratinocyte cells for herpes simplex virus type 2 early infection.Meth-ods:The herpes simplex virus type 2 were used to infect the human immortalized keratinocyte cells,and de-tected the IL-1 9 and IL-20mRNA (messenger RNA)expression levels after 24,48,72 hours after infection using fluorescence real-time PCR,then analyzed the influence of HSV-2 on HaCaT cells secreting interleukin (IL)-1 9 and IL-20.Results:The HSV-2 for HaCaT cells secreting IL-1 9 and IL-20mRNA expression levels of experimental group was significantly higher than that of control group (P <0.05).Conclusion:Keratinocytes infected by herpes simplex virus type 2 can secrete IL-1 9 and IL-20,and they participate in the immune de-fense response in herpes simplex type virus 2 early infection.