The extracellular polymeric substance (EPS) was secreted when marine benthic diatom attached on submerged surfaces,the environment factors had a directly impact on the composition and secretion action of EPS.The effects of the oxygen content and the attachment area on EPS were studied.The EPS from marine benthic diatoms were extracted by hot solvent method.The phenol-sulfuric acid method was employed to measure the content of polysaccharide and the Coomassie Brilliant Blue G-250 method was employed to measure the content of protein in the extractions.The cell number of diatoms was measured through the method of blood cell counting plate and UV spectrophotometry.The results show that the growth rate of diatoms is fast and the secretion amounts of EPS in a single cell are great under the higher oxygen content and larger attachment area conditions.The oxygen content has a great impact on the content of water-soluble polysaccharide as the main composition in EPS,while it has little impact on the else components.Compared to the oxygen content,the attachment area has little impact on the attachment and growth of diatoms.%胞外多聚物(extracellular polymeric substance,EPS)是海洋底栖硅藻在水下表面附着时分泌的物质,环境因素对胞外多聚物的组成和分泌行为有直接影响,本文重点探讨了氧含量和附着面积对胞外多聚物的影响.采用热溶剂浸提法提取硅藻胞外多聚物,利用苯酚-硫酸法定量其中的多糖成分,利用考马斯亮蓝G-250法定量蛋白质成分,通过血球计数板法和紫外分光光度法计算细胞数量.结果表明,在氧含量高、可附着面积大的条件下,硅藻的生长繁殖速率快,且单个细胞的胞外多聚物分泌量大.氧含量对胞外多聚物主要成分中的水溶性多糖含量影响大,而对其余成分含量影响小.可附着面积对硅藻附着和生长的影响比氧含量影响小.
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机译:用于啤酒的二次过滤的过滤材料及其生产方法技术领域本发明涉及一种用于包含硅藻土的啤酒的二次过滤的过滤材料及其制备方法,其中在与过滤的啤酒的流动相反的方向上,所述过滤材料为由第一层粗过滤硅藻土制成,其厚度大于2毫米,过滤面积的范围为0.66至1.05 kg / me2,第二层由混合物制成,厚度大于2毫米过滤面积为0.55至1.20 kg / me2的细过滤硅藻土和过滤面积为0.10至0.20 kg / me2的吸附材料,第三过滤层具有粗滤硅藻土和细滤硅藻土的重量比为10:90至30:70的混合物产生的厚度大于1.5mm。