大鼠急性脑缺血再灌注损伤中康络素对谷氨酸抑制作用的实验研究

摘要

目的：探讨康络素对脑缺血再灌注损伤的保护作用。方法：采用Pulsinelli的四血管闭塞法制备大鼠急性脑缺血再灌注模型，利用高压液相色谱法观察脑缺血再灌注期间谷氨酸、γ-氨基丁酸含量变化，通过图象分析仪测定神经元的面数密度及康络素对以上指标的影响。结果：谷氨酸、γ-氨基丁酸含量于缺血后明显升高，再灌注后降低，康络素可降低谷氨酸含量，与盐水对照组相比差异显著(P<0.01)，同时增加神经元面数密度。结论：康络素通过抑制谷氨酸释放可减轻脑缺血再灌注期间脑组织损伤。%Objective:To study the Cronassial′protective effect on brain tissue during ischemia reperfusion.Methods:The rat cerebral ischemia reperfusion model was prepared by four-vessel occulasion method.The Glu,GABA content in hippocampus during cerebral ischemia reperfusion was observed by high press liquid phase method.Results:Glu,GABA content increased during ischemia and decreased during reperfusion. Cronassial could obviously decrease the Glu content and increase neuron density.Conclusion:Cronassial can inhibit Glu and protect brain tissue during cerebral ischemia reperfusion.