To compare the immunity in developmental stage of insect, the dynamics of phenoloxidase ac tivity in vitro during different developmental stage of Locusta migratoria manilensis Meyen were tested and analyzed. The results showed as follow: During the development, the phenoloxidase activity arrived at the highest under 25 ℃ but in female adults it reached its peak at 30 ℃ . Under different temperature, there were similar changes from the 1" to the 5th larvae. There was the lowest phenoloxidase activity in the 1st larvae and the highest phenoloxidase activity in the 5th larvae. In adult, the phenoloxidase activity in fe male adult was higher than that in male adult under 30℃ and 45℃. The dynamics of phenoloxidase ac tivity of Locusta migratoria manilensis Meyen during different developmental stages under different temperature proved to be different in immunocompetence and provided references for the target on phenoloxidase activity to develop biological insecticide.%为比较昆虫发育时期免疫防御能力的强弱,对不同发育时期东亚飞蝗体外酚氧化酶活性的动态变化进行了测定和比较分析.结果显示:从东亚飞蝗不同发育时期看,1～5龄蝗蝻及雄成虫25℃时酚氧化酶活性达到峰值,雌成虫30℃时酚氧化酶活性达到峰值,雌虫酶活性高于雄虫；不同温度作用下,1～5龄蝗蝻在15～45℃温度变化中变化规律相似,1龄蝗蝻酶活性最低,2～4龄蝗蝻时期酶活性升高,5龄蝗蝻时酶活性达到蜂值,成虫酶活性在30℃和45℃时,雌虫酶活性高于雄虫(P＜0.05).东亚飞蝗不同发育时期、不同温度作用下酚氧化酶活性动态变化显示了其免疫能力的强弱差异.
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