以玉米秸秆粗纤维降解率为评价指标,通过单因素试验考察了pH、酶解温度、酶解时间和酶浓度对玉米秸秆酶解后粗纤维变化的影响,并在单因素基础上通过响应面试验优化了玉米秸秆酶解工艺参数,确定了玉米秸秆最佳酶解工艺:pH 5.0、酶解温度55℃、酶解时间60 h,酶浓度50 FPU/g,在此酶解条件下,玉米秸秆粗纤维降解率达到了45.62％.%With fiber degradation rate of maize stalks as evaluating index,the effects of pH,enzymolysis time,enzymolysis temperature and enzyme concentration on fiber degradation rate were investigated by single factor test.According to the single factor test,the process was optimized through Response Surface Experiment and the best enzymolysis conditions were obtained.Under these enzymolysis conditions,the fiber degradation rate of maize stalks after enzymolysis was 45.62％ at the conditions of the pH 5.0,enzymolysis time 60 h,enzymolysis temperature 55 ℃ and enzyme concentration 50 FPU/g.
展开▼
