肠炎沙门氏菌血清型特异性基因挖掘及生物信息学分析

摘要

肠炎沙门氏菌(Salmonella enterica serovar Enteritidis)能引起畜禽的胃肠炎以及食物中毒，其血清型鉴定较为复杂。为了挖掘肠炎沙门氏菌血清型特异性基因，以44个已测序沙门氏菌基因组构建数据库，利用肠炎沙门氏菌所有蛋白编码序列为查询序列，通过BLASTN比对，挖掘出6个肠炎沙门氏菌血清型特异性基因（SEN1382, SEN1383, SEN1388, SEN1936, SEN1945和SEN1959），其中4个基因编码噬菌体相关蛋白。同时，研究分析了肠炎沙门氏菌血清型特异性基因的理化性质、二级结构、三级结构、亚细胞定位、信号肽和跨膜区等特征，结果表明肠炎沙门氏菌血清型特异性基因具有多样性特征。挖掘的血清型特异性基因可为鉴定肠炎沙门氏菌提供检测靶标。%Salmonella enterica serovar Enteritidis is one of the major causes of gastroenteritis and food poisoning in humans due to the consumption of poultry derivatives. To mine serovar-specific genes of S. enterica serovar Enteritidis, all CDSs of it were searched in a searching database of 44 other Salmonella genomic sequences using SMM-system tool. Six serovar-specific genes were identified including SEN1382, SEN1383, SEN1388, SEN1936, SEN1945, and SEN1959. Among them, four genes decoded phage-related proteins. The characteristics of these genes were analyzed including physicochemical properties, secondary structure, three-dimensional structure, signal peptide, transmembrane regions, and subcellular localization. The results indicated the diversification of these genes. These serovar-specific genes can be used as the targets for detecting and identifying S. enterica serovar Enteritidis.