辛伐他汀对老年大鼠心肌细胞白细胞介素-1β和肿瘤坏死因子-α表达的影响

摘要

目的：观察辛伐他汀对老年大鼠心肌细胞中炎性因子白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）表达的影响。方法以24月龄大鼠为实验对象，按常规方法分离、剪碎并消化心室肌组织，以含10%胎牛血清的DMEM培养心肌细胞。将培养细胞分为衰老模型对照组、溶剂对照组（DMSO）和辛伐他汀1和10μmol/L组，各组细胞经相应处理后，分别用RT-PCR和Western印迹法检测IL-1β和TNF-α的mRNA及蛋白表达水平，并比较其变化。结果 RT-PCR结果显示，与衰老模型组相比，溶剂组IL-1β和TNF-αmRNA表达无明显变化（P＞0.05），辛伐他汀1和10μmol/L组IL-1β和TNF-αmRNA表达均明显降低（分别为P＜0.05和P＜0.01）；与溶剂对照组相比，辛伐他汀1和10μmol/L组IL-1β和TNF-αmRNA表达均明显降低（分别为P＜0.05和P＜0.01）；Western印迹法结果显示，与衰老模型组相比，溶剂对照组IL-1β蛋白表达无明显变化，TNF-α蛋白表达明显降低（P＜0.01），辛伐他汀1和10μmol/L组IL-1β和TNF-α蛋白表达均明显降低（P＜0.01）；与溶剂对照组相比，辛伐他汀1和10μmol/L组IL-1β蛋白表达均明显降低（P＜0.01），而TNF-α蛋白表达无明显变化（P＞0.05）。结论辛伐他汀可下调衰老大鼠心肌细胞炎性因子IL-1β和TNF-α的基因表达。%Objective To investigate the effect of simvastatin on the expression of interleukin-1β(IL-1β) and tumor necrosis factorα(TNF-α) in cardiomyocyte of aging rat. Methods Primary cultures of cardiomyocyte were got from aging rats. Myocyte were divided into control group, dimerthyl sulfoxide(DMSO) group, and simvastatin group, which were treated respectively by cell culture medium, DMSO and simvastatin (1 and 10 μmol/L). The expression of IL-1β、 TNF-α mRNA was evalulated by RT-PCR, and contents of IL-1βand TNF-αprotein were detected by Western blot. Results RT-PCR showed that, compared with the aging model group, IL-1β and TNF-α mRNA expression of solvent group was no significant change (P>0.05), the mRNA expression of IL-1βand TNF-αin 1 and 10μmol/L simvastatin group was significantly lower (P0.05). Conclusions Simvastatin down-regulates IL-1βand TNF-αgene expression.