新型2-吲哚酮类c-Met激酶抑制剂的设计、合成及活性研究

摘要

目的 设计合成新型2-吲哚酮类c-Met激酶抑制剂.方法 以c-Met激酶抑制剂SU11274为先导化合物,利用生物电子等排原理设计出系列2-吲哚酮类衍生物.以2-吲哚酮为起始原料,先后经历与氯磺酸的氯磺酰化、与3的磺酰胺化、与6a~6h,7a~7h和4a~4b的缩合反应制得目标产物10a~10r,并测定它们对c-Met激酶和MCF-7细胞增殖的抑制活性.结果与结论 成功合成了设计的18个2-吲哚酮类化合物,产物结构经1H NMR和ESI-MS确证.部分化合物显示出一定的c-Met激酶和MCF-7细胞增殖抑制活性.对目标产物进行了初步构效关系分析,为该类化合物进一步的结构优化奠定了基础.%Objective To design and synthesize novel 2-indolone derivatives as the c-Met kinase inhibitors. Methods With c-Met kinase inhibitor SU11274 as lead compound,a series of 2-indolone derivatives were designed according to the concept of bioiso-sterism. Then the target compounds(10a-10r)were synthesized from 2-indolone through 5-chlorosulfonation with chlorosulfonic acid, sulfonamidation with intermediate 3,condensation with 6a-6h,7a-7h and 4a-4b,respectively. Their inhibitory activity against c-Met and proliferation of MCF-7 cells were evaluated. Results and Conclusion The designed compounds were successfully prepared and their structures were confirmed by 1H NMR and ESI-MS. Some compounds had certain inhibitory activity against c-Met and prolif-eration of MCF-7 cells. An initial structure-activity relationship analysis of these compounds was performed to provide useful informa-tion for further optimization of their structures.